Author(s):
Spera, Kamille D. [UNESP] ; Figueiredo, Patrícia A. [UNESP] ; Santos, Pamela C. E. [UNESP] ; Barbosa, Fernando C. [UNESP] ; Alves, Caio P. [UNESP] ; Dokkedal, Anne Lígia [UNESP] ; Saldanha, Luiz Leonardo [UNESP] ; Silva, Luciana P. ; Figueiredo, Carlos Rogério ; Ferreira, Paulo Cesar [UNESP] ; Da Silva, Regildo M. G. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201089
Origin: Oasisbr
Subject(s): B16F10 melanoma cells; Chromosomal aberration; DPPH radical scavenging; HPLC-DAD; Micronucleus test; B16F10 melanoma cells; B16F10 melanoma cells; Chromosomal aberration; Chromosomal aberration; DPPH radical scavenging; DPPH radical scavenging; HPLC-DAD; HPLC-DAD; Micronucleus test; Micronucleus test
Description
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Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.
Universidade Estadual Paulista/UNESP Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia Laboratório de Fitoterápicos e Produtos, Naturais, Av. Dom Antônio, 2100
Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista/UNESP, Av. Eng. Luís Edmundo Carrijo Coube, 2085, Núcleo Residencial Presidente Geisel
Fundação Educacional do Município de Assis/FEMA, Av. Getulio Vargas, 1200, Vila Nova Santana
Departamento de Microbiologia Imunologia e Parasitologia Divisão de Biologia Celular Unidade de Oncologia Experimental/ UNONEX Universidade Federal de São Paulo/UNIFESP, Rua Botucatu, 740, Vila Clementino
Universidade Estadual Paulista/UNESP Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia Laboratório de Fitoterápicos e Produtos, Naturais, Av. Dom Antônio, 2100
Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista/UNESP, Av. Eng. Luís Edmundo Carrijo Coube, 2085, Núcleo Residencial Presidente Geisel
