Author(s):
Herrera, Naiara A. [UNESP] ; Duchatsch, Francine [UNESP] ; Tardelli, Lidieli P. [UNESP] ; Dionísio, Thiago J. ; Shinohara, Andre L. ; Santos, Carlos F. ; Amaral, Sandra Lia [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201101
Origin: Oasisbr
Subject(s): Arterial pressure; Glucocorticoids; Microcirculation; Physical training; Skeletal muscle; Arterial pressure; Arterial pressure; Glucocorticoids; Glucocorticoids; Microcirculation; Microcirculation; Physical training; Physical training; Skeletal muscle; Skeletal muscle
Description
Made available in DSpace on 2020-12-12T02:24:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-05
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and prevent DEX-induced microvascular rarefaction. Rats underwent training for 8 weeks and then were treated with DEX (50 μg/kg per day, s.c.) for 14 days. Arterial pressure was measured and tibialis anterior (TA) muscle was collected for analyses. DEX induced hypertension concomitantly with capillary density loss (CD, −23.9%) and decrease of VEGF (−43.0%), p-AKT/AKT (−39.6%) and Bcl-2 (−23.0%) and an increase in caspase-3-cleaved protein level (+34.0%) in TA muscle. Training upregulated microRNA-126 expression (+13.1%), prevented VEGF (+61.4%), p-AKT/AKT (+37.7%), Bcl-2 (+7.7%) decrease and caspase-3-cleaved (−23.1%) increase associated with CD (+54.7%) reduction and hypertension prevention. MiRNA-126 upregulation, induced by training, plays a role in controlling microcirculation, which may be a potential target against DEX-induced microvascular rarefaction.
Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho 676
Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75
Department of Physical Education - São Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa
Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho 676
Department of Physical Education - São Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa
FAPESP: 2015/03965-2
FAPESP: 2016/12532-5
FAPESP: 2017/00509-1
FAPESP: 2017/14405-3
FAPESP: 2018/06998-7
CAPES: 88882.426901/2019-01
CAPES: 88882.426908/2019-01
