Author(s):
Dias, Regiane C. B. [UNESP] ; Tanabe, Rodrigo H. S. [UNESP] ; Vieira, Melissa A. [UNESP] ; Cergole-Novella, Maria C. ; dos Santos, Luís F. ; Gomes, Tânia A. T. ; Elias, Waldir P. ; Hernandes, Rodrigo T. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201123
Origin: Oasisbr
Subject(s): antimicrobial resistance; diagnosis; diarrhea; EAEC; virulence factors; antimicrobial resistance; antimicrobial resistance; diagnosis; diagnosis; diarrhea; diarrhea; EAEC; EAEC; virulence factors; virulence factors
Description
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Enteroaggregative Escherichia coli (EAEC) is an important agent of acute and persistent diarrhea in children and adults worldwide. Here we report a characterization of 220 EAEC isolates, 88.2% (194/220) of which were typical and 11.8% (26/220) were atypical, obtained from diarrheal patients during seven years (2010-2016) of epidemiological surveillance in Brazil. The majority of the isolates were assigned to phylogroups A (44.1%, 97/220) or B1 (21.4%, 47/220). The aggregative adherence (AA) pattern was detected in 92.7% (204/220) of the isolates, with six of them exhibiting AA concomitantly with a chain-like adherence pattern; and agg5A and agg4A were the most common adhesin-encoding genes, which were equally detected in 14.5% (32/220) of the isolates. Each of 12 virulence factor-encoding genes (agg4A, agg5A, pic, aap, aaiA, aaiC, aaiG, orf3, aar, air, capU, and shf) were statistically associated with typical EAEC (P < 0.05). The genes encoding the newly described aggregate-forming pili (AFP) searched (afpB, afpD, afpP, and afpA2), and/or its regulator (afpR), were exclusively detected in atypical EAEC (57.7%, 15/26), and showed a significant association with this subgroup of EAEC (P < 0.001). In conclusion, we presented an extensive characterization of the EAEC circulating in the Brazilian settings and identified the afp genes as putative markers for increasing the efficiency of atypical EAEC diagnosis.
Universidade Estadual Paulista (UNESP) Instituto de Biociências
Laboratório Regional de Santo André Instituto Adolfo Lutz
Centro de Bacteriologia Instituto Adolfo Lutz
Departamento de Microbiologia Imunologia e Parasitologia Universidade Federal de São Paulo- Escola Paulista de Medicina
Laboratório de Bacteriologia Instituto Butantan
Universidade Estadual Paulista (UNESP) Instituto de Biociências
