Author(s):
Bocato, Mariana Zuccherato ; Cesila, Cibele Aparecida ; Lataro, Beatriz Favero ; de Oliveira, Anderson Rodrigo Moraes [UNESP] ; Campíglia, Andres Dobal ; Barbosa Jr, Fernando
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201149
Origin: Oasisbr
Subject(s): Endocrine disruptors; Human urine; Liquid chromatography-tandem mass spectrometry; Multiclass analysis; Vortex-assisted dispersive liquid-liquid microextraction; Endocrine disruptors; Endocrine disruptors; Human urine; Human urine; Liquid chromatography-tandem mass spectrometry; Liquid chromatography-tandem mass spectrometry; Multiclass analysis; Multiclass analysis; Vortex-assisted dispersive liquid-liquid microextraction; Vortex-assisted dispersive liquid-liquid microextraction
Description
Made available in DSpace on 2020-12-12T02:25:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Simplicity, speed, and reduced cost are essential demands for routine analysis in human biomonitoring studies. Moreover, the availability of higher volumes of human specimens is becoming more restrictive due to ethical controls and to the costs associated with sample transportation and storage. Thus, analytical methods requiring much lower sample volumes associated with simultaneous detection capability (multiclass analysis) are with a very high claim. In this sense, the present approach aimed at the development of a method for preconcentration and simultaneous determination of four classes of endocrine disruptors (seven bisphenols, seven parabens, five benzophenones, and two antimicrobials) in the urine. The approach is based on vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). After optimization of the significant parameters of VADLLME extraction, the proposed procedure showed to be simple, fast, sensitive, requiring only 1.0 mL of urine, 400 μL of organic solvents with a total stirring time of 20 s. Moreover, a variation of inter-day and between-day runs were lower than 10.0% and 11.0%, respectively. Finally, the proposed method was successfully applied to the analysis of 50 urine samples of Brazilian pregnant women to establish reference ranges.
Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
Faculdade de Ciências Farmacêuticas Universidade de Ribeirão Preto
Departamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
Department of Chemistry University of Central Florida, P.O.Box 25000
National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
FAPESP: 14/50867–3
CNPq: 154866/2018–0
FAPESP: 2014/19914–5
FAPESP: 2014/50945–4
FAPESP: 2015/20725–5
FAPESP: 2018/07534–4
FAPESP: 2018/24069–3
CNPq: 309822/2017–3
CNPq: 422588/2016–4
CNPq: 465389/2014–7
CNPq: 465571/2014–0
CAPES: 88887.370750/2019–00
