Author(s):
Pereira Scarpa, Josiani de Cassia [UNESP] ; Paganini Marques, Natália [UNESP] ; Alves Monteiro, Diego [UNESP] ; Martins, Gisele Marta [UNESP] ; Paula, Ariela Veloso de [UNESP] ; Boscolo, Maurício [UNESP] ; da Silva, Roberto [UNESP] ; Gomes, Eleni [UNESP] ; Alonso Bocchini, Daniela [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201202
Origin: Oasisbr
Subject(s): Cellulosic ethanol; Enzymatic saccharification; Lignocellulases; Pretreatment; Solid-state fermentation; Cellulosic ethanol; Cellulosic ethanol; Enzymatic saccharification; Enzymatic saccharification; Lignocellulases; Lignocellulases; Pretreatment; Pretreatment; Solid-state fermentation; Solid-state fermentation
Description
Made available in DSpace on 2020-12-12T02:26:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01
Pycnoporus sanguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 °C. The highest endoglucanase, exoglucanase, β-glucosidase, xylanase, β-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g−1, respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g−1 for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L−1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 °C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained.
São Paulo State University (UNESP) Institute of Biosciences Letters and Exact Sciences
São Paulo State University (UNESP) Institute of Chemistry
São Paulo State University (UNESP) Faculty of Pharmaceutical Sciences
São Paulo State University (UNESP) Institute of Biosciences Letters and Exact Sciences
São Paulo State University (UNESP) Institute of Chemistry
São Paulo State University (UNESP) Faculty of Pharmaceutical Sciences
