Author(s):
Tamayo, Abel I. Balbin ; Rizo, Laura S. López ; de Armas, Madelin Blanco ; Ferreira, Antonio Aparecido Pupim [UNESP] ; Manzani, Danilo ; Yamanaka, Hideko [UNESP] ; Guas, Ana Margarita Esteva
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201371
Origin: Oasisbr
Subject(s): DNA biosensors; Genosensor; HIV-1; Hybridization; Impedimetric; Proviral DNA; DNA biosensors; DNA biosensors; Genosensor; Genosensor; HIV-1; HIV-1; Hybridization; Hybridization; Impedimetric; Impedimetric; Proviral DNA; Proviral DNA
Description
Made available in DSpace on 2020-12-12T02:30:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01
This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.
Department of Analytical Chemistry University of Havana
AIDS Research Laboratory (LISIDA)
Department of Analytical Chemistry São Paulo State University (UNESP)
São Carlos Institute of Chemistry Department of Chemistry and Molecular Physics University of São Paulo (USP)
Department of Analytical Chemistry São Paulo State University (UNESP)
