Author(s):
de Souza, Eline Almeida Rodrigues ; de Carvalho Araujo, Andreina ; Pires, Larissa Célly Souza Regis ; Freschi, Carla Roberta [UNESP] ; Azevedo, Sergio Santos ; Machado, Rosangela Zacarias [UNESP] ; Horta, Maurício Claudio
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201373
Origin: Oasisbr
Subject(s): Babesia caballi; Diagnostic; ELISA; Horse; PCR; Theileria equi; Babesia caballi; Babesia caballi; Diagnostic; Diagnostic; ELISA; ELISA; Horse; Horse; PCR; PCR; Theileria equi; Theileria equi
Description
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Equine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P < 0.05) were: Purebred (P < 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil.
Laboratório de Doenças Parasitárias Universidade Federal do Vale do São Francisco – UNIVASF
Universidade Federal de Minas Gerais – UFMG
Faculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista Júlio de Mesquita Filho – UNESP
Unidade Acadêmica de Medicina Veterinária Universidade Federal de Campina Grande – UFCG
Faculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista Júlio de Mesquita Filho – UNESP
