Autor(es):
Leonel, Ellen Cristina Rivas [UNESP] ; Campos, Silvana Gisele Pegorin ; Guerra, Luiz Henrique Alves [UNESP] ; Bedolo, Carolina Marques [UNESP] ; Vilamaior, Patrícia Simone Leite [UNESP] ; Calmon, Marilia Freitas [UNESP] ; Rahal, Paula [UNESP] ; Amorim, Christiani Andrade ; Taboga, Sebastião Roberto [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/201374
Origem: Oasisbr
Assunto(s): Estrogen; Estrogen receptor; Mammary gland; Mongolian gerbil; Progesterone receptor; Xenoestrogen; Estrogen; Estrogen; Estrogen receptor; Estrogen receptor; Mammary gland; Mammary gland; Mongolian gerbil; Mongolian gerbil; Progesterone receptor; Progesterone receptor; Xenoestrogen; Xenoestrogen
Descrição
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Hormonal regulation controls mammary gland (MG) development. Therefore some hormone-related factors can disrupt the early phases of MGs development, making the gland more susceptible to long term modifications in its response to circulating hormones. Endocrine disruptors, such as bisphenol A (BPA), are able to cause alterations in hormone receptor expression, leading to changes in the cell proliferation index, which may expose the tissue to neoplastic alterations. Thus, we evaluated the variations in hormone receptor expression in the MG of 6-month old Mongolian gerbils exposed to BPA and 17β estradiol during the perinatal period. Receptors for estrogen alpha (ERα), beta (ERβ), progesterone (PGR), prolactin (PRL-R), and co-localization of connexin 43 (Cx43) and ERα in gerbils were analyzed, and serum concentrations of estradiol and progesterone were assessed. No alterations in body, liver, and ovary-uterus complex weights were observed. However, there was an increase in epithelial ERα expression in the 17β estradiol (E2) group and in PGR in the BPA group. Although immunohistochemistry did not show alterations in ERβ expression, western blotting revealed a decrease in this protein in the BPA group. PRL-R was more present in epithelial cells in the vehicle control (VC), E2, and BPA groups in comparison to the intact control group. Cx43 was more frequent in E2 and BPA groups, suggesting a protective response from the gland against possible malignancy. Serum concentration of estradiol reduced in VC, E2, and BPA groups, confirming that alterations also impacts steroid levels. Consequently, perinatal exposure to BPA and the reference endogenous estrogen, 17β estradiol, are able to increase the tendency of endocrine disruption in MG in a long term manner, since repercussions are observed even 6 months after exposure.
Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim Nazareth
Federal University of São João del Rei (UFSJ) Campus Centro Oeste Dona Lindu, Avenida Sebastião Gonçalves Coelho, 400, Bairro Chanadour
Laboratory of Gynecology Institute of Experimental and Clinique Research Université Catholique de Louvain (UCL), Avenue Mounier 52, Bte B1.52.02
Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim Nazareth
CAPES: 001
FAPESP: 2015/01548–5
FAPESP: 2016/22947–8
CNPq: 305840/2015–0
