Author(s):
Maistro, Edson Luis [UNESP] ; Terrazzas, Peterson Menezes [UNESP] ; Perazzo, Fábio Ferreira ; Gaivão, Isabel O’Neill De Mascarenhas ; Sawaya, Alexandra Christinie Helena Frankland ; Rosa, Paulo Cesar Pires
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201472
Origin: Oasisbr
Subject(s): Salicaceae toxicity; Salix alba bark extract genotoxicity; Salix comet assay; Salix micronucleus test; White willow genotoxicity; Salicaceae toxicity; Salicaceae toxicity; Salix alba bark extract genotoxicity; Salix alba bark extract genotoxicity; Salix comet assay; Salix comet assay; Salix micronucleus test; Salix micronucleus test; White willow genotoxicity; White willow genotoxicity
Description
Made available in DSpace on 2020-12-12T02:33:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-17
Salix alba (SA), commonly known as white willow, is a plant used in folk medicine for the treatment of chronic and acute inflammation, infection, pain, and fever. The phytochemical characterization of the bark extract of this plant indicated that its main component is salicin, a precursor of the anti-inflammatory agent acetylsalicylic acid. Considering the lack of studies evaluating the genetic toxicity and cytotoxic action of SA bark extract on human cells, as well as the chemical characterization of its major phenolic compounds, the present study was designed to (1) investigate the cytotoxic and genotoxic potential of SA bark extract on human peripheral leukocyte cells and human hepatoma cell line HepG2, and (2) characterize its major phenolic constituents. The phenolic compounds found were salicylic acid, salicin, salidroside, saligenin, tremulodin, salicoylsalicin, salicortin, and tremulacin. The results using trypan blue staining test showed viability decreases (viability less than 70%) for concentrations of SA extract equal and higher to 200 µg/ml. Low genotoxic activity (comet assay) was exhibited for 50 and 100 µg/ml SA extract in human leukocytes. SA did not exert a marked clastogenic/aneugenic effect on leukocytes and HepG2 human cells. Data suggest that the genotoxic effects of SA bark extract occur when it is not metabolized by liver enzymes.
Speech and Hearing Therapy Department São Paulo State University - UNESP
Programa de Pós-Graduação em Biologia Geral e Aplicada São Paulo State University–UNESP Instituto de Biociências
Institute of Environmental Chemical and Pharmaceutical Sciences Federal University of São Paulo
Department of Genetics and Biotechnology and Animal and Veterinary Research Centre (CECAV) University of Trás-os-Montes and Alto Douro (UTAD)
Faculty of Pharmaceutical Sciences University of Campinas
Speech and Hearing Therapy Department São Paulo State University - UNESP
Programa de Pós-Graduação em Biologia Geral e Aplicada São Paulo State University–UNESP Instituto de Biociências
