Autor(es):
de Oliveira, Rosa Virginia Dutra [UNESP] ; Bonafé, Fernanda Salloume Sampaio [UNESP] ; Spolidorio, Denise Madalena Palomari [UNESP] ; Koga-ito, Cristiane Yumi [UNESP] ; de Farias, Aline Leite [UNESP] ; Kirker, Kelly R. ; James, Garth A. ; Brighenti, Fernanda Lourenção [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/201539
Origem: Oasisbr
Assunto(s): A; Biofilm; Dental caries; Microbial interaction; Mutans; Naeslundii; S; Virulence factor; A; A; Biofilm; Biofilm; Dental caries; Dental caries; Microbial interaction; Microbial interaction; Mutans; Mutans; Naeslundii; Naeslundii; S; S; Virulence factor; Virulence factor
Descrição
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
The study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize—with an emphasis on biofilm formation and composition and metabolic activity—single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single-and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide—“XTT”—reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans’ resistance to chlorhexidine.
School of Dentistry São Paulo State University (UNESP)
São José dos Campos Institute of Science and Technology São Paulo State University (UNESP)
Centre for Biofilm Engineering Montana State University
School of Dentistry São Paulo State University (UNESP)
São José dos Campos Institute of Science and Technology São Paulo State University (UNESP)
CAPES: 001
FAPESP: 2012/17236-4
FAPESP: 2013/12326-8
FAPESP: 2014/02397-8
FAPESP: 2014/16935-1
FAPESP: 2015/07679-4
CNPq: 304383/2016-3
