Author(s): Araujo, Heitor Ceolin [UNESP] ; Nakamune, Ana Cláudia Melo Stevanato [UNESP] ; Garcia, Wilson Galhego [UNESP] ; Pessan, Juliano Pelim [UNESP] ; Antoniali, Cristina [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201557
Origin: Oasisbr
Made available in DSpace on 2020-12-12T02:35:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage.
Graduate Program in Dental Science São Paulo State University (UNESP) School of Dentistry
Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry
Multicenter Graduate Program in Physiological Sciences São Paulo State University (UNESP) School of Dentistry
Department of Basic Sciences São Paulo State University (UNESP) School of Dentistry
Graduate Program in Dental Science São Paulo State University (UNESP) School of Dentistry
Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry
Multicenter Graduate Program in Physiological Sciences São Paulo State University (UNESP) School of Dentistry
Department of Basic Sciences São Paulo State University (UNESP) School of Dentistry
