Autor(es):
Duarte, Luis Felipe de Almeida ; Blasco, Julián ; Catharino, Marília Gabriela Miranda ; Moreira, Edson Gonçalves ; Trombini, Chiara ; Nobre, Caio Rodrigues ; Moreno, Beatriz Barbosa ; Abessa, Denis Moledo de Souza [UNESP] ; Pereira, Camilo Dias Seabra
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/201596
Origem: Oasisbr
Assunto(s): Biomarkers; Cytotoxicity; Genotoxicity; Lead; Subcellular metal partitioning; Ucides cordatus; Biomarkers; Biomarkers; Cytotoxicity; Cytotoxicity; Genotoxicity; Genotoxicity; Lead; Lead; Subcellular metal partitioning; Subcellular metal partitioning; Ucides cordatus; Ucides cordatus
Descrição
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Lead is a priority pollutant introduced in the aquatic environment by different sources commonly located in estuarine regions, such as ports, marinas and industries. Environmental agencies around the world set the maximum allowable concentration of lead in effluents, surface water and sediment, but few studies reported its accumulation and chronic toxicity in mangrove benthic invertebrates using concentrations believed to be safe. In the case of Brazilian mangrove environments, Ucides cordatus is a crab species of choice to be used in bioaccumulation studies. We have assessed biomarkers’ responses (DNA strand breaks, micronucleated cells, metallothioneins, enzymatic activity of aminolevulinic acid dehydratase and neutral red retention time) and the total bioaccumulation in six tissues of U. cordatus crabs resident to mangrove areas under different conservation status during a 28-day period bioassay. We also investigated Pb subcellular partition and biomarkers’ responses using a supposedly safe concentration (10 μg L−1). During the Pb exposure, the highest concentration of Pb was observed in crab gills. Crabs also showed a high ability to allocate Pb in detoxified forms. Multivariate analysis pointed out that bioaccumulation (total, active and detoxified) is linked to biomarkers. Even in supposedly safe dosage, U. cordatus triggered its defense mechanisms expressing more metallothioneins and presented relevant cyto-genotoxic damage. Our data suggest the development of biological tolerance to Pb in crabs from polluted areas. Our results provided a new insight about lead toxicity even at concentrations considered environmentally safe, which could support new strategies to manage estuarine areas considering their respective conservation status.
Universidade Federal de São Paulo Departamento de Ciências do Mar Campus Baixada Santista, Rua Maria Máximo, 168, Ponta da Praia
Instituto de Ciencias Marinas de Andalucía (ICMAN), Campus Río San Pedro, s/n, Puerto Real
Instituto de Pesquisas Energéticas e Nucleares (IPEN – CNEN/SP) Cidade Universitária, Av. Professor Lineu Prestes, 2242
Universidade Estadual de São Paulo – UNESP Center of Investigation on Aquatic Pollution and Ecotoxicology, Praça Infante Dom Henrique, s/n
Universidade Santa Cecília Laboratório de Ecotoxicologia, Rua Oswaldo Cruz, 266
Universidade Estadual de São Paulo – UNESP Center of Investigation on Aquatic Pollution and Ecotoxicology, Praça Infante Dom Henrique, s/n
FAPESP: 2010/01552-9
FAPESP: 2014/13448-2
FAPESP: 2016/05414-6
