Autor(es):
Rath-Deschner, Birgit ; Nogueira, Andressa Vilas Boas ; Memmert, Svenja ; Nokhbehsaim, Marjan ; Cirelli, Joni Augusto [UNESP] ; Eick, Sigrun ; Miosge, Nicolai ; Kirschneck, Christian ; Kesting, Marco ; Deschner, James ; Jäger, Andreas ; Damanaki, Anna
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/207106
Origem: Oasisbr
Assunto(s): Fusobacterium nucleatum; Gingivitis; Orthodontic tooth movement; Periodontal ligament; Periodontitis; Periodontium; Fusobacterium nucleatum; Fusobacterium nucleatum; Gingivitis; Gingivitis; Orthodontic tooth movement; Orthodontic tooth movement; Periodontal ligament; Periodontal ligament; Periodontitis; Periodontitis; Periodontium; Periodontium
Descrição
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Deutscher Akademischer Austauschdienst
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Deutsche Forschungsgemeinschaft
The aim of the study was to clarify whether orthodontic forces and periodontitis interact with respect to the anti-apoptotic molecules superoxide dismutase 2 (SOD2) and baculoviral IAP repeat-containing protein 3 (BIRC3). SOD2, BIRC3, and the apoptotic markers caspases 3 (CASP3) and 9 (CASP9) were analyzed in gingiva from periodontally healthy and periodontitis subjects by real-time PCR and immunohistochemistry. SOD2 and BIRC3 were also studied in gingiva from rats with experimental periodontitis and/or orthodontic tooth movement. Additionally, SOD2 and BIRC3 levels were examined in human periodontal fibroblasts incubated with Fusobacterium nucle-atum and/or subjected to mechanical forces. Gingiva from periodontitis patients showed significantly higher SOD2, BIRC3, CASP3, and CASP9 levels than periodontally healthy gingiva. SOD2 and BIRC3 expressions were also significantly increased in the gingiva from rats with experimental periodontitis, but the upregulation of both molecules was significantly diminished in the concomitant presence of orthodontic tooth movement. In vitro, SOD2 and BIRC3 levels were significantly increased by F. nucleatum, but this stimulatory effect was also significantly inhibited by mechanical forces. Our study suggests that SOD2 and BIRC3 are produced in periodontal infection as a protective mechanism against exaggerated apoptosis. In the concomitant presence of orthodontic forces, this protective anti-apoptotic mechanism may get lost.
Department of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn
Department of Periodontology and Operative Dentistry University Medical Center University of Mainz
Department of Orthodontics and Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of Bonn
Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of Bonn
Department of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESP
Department of Periodontology Laboratory for Oral Microbiology zmk bern Zahnmedizinische Kliniken
Department of Prosthodontics University Medical Center
Department of Orthodontics University of Regensburg
Department of Oral and Maxillofacial Surgery Friedrich-Alexander University Erlangen-Nürnberg (FAU)
Department of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESP
FAPESP: 2017/07137-2
Deutscher Akademischer Austauschdienst: 57391253
CAPES: 88881.144012/2017-01
Deutsche Forschungsgemeinschaft: DE1593/5-1
