Author(s):
Castilho, Maíra Sales [UNESP] ; Rahal, Sheila Canevese [UNESP] ; Dias Neto, Ramiro das Neves [UNESP] ; Pereira, Ana Cristina ; Francia, Camila Contin Diniz de Almeida [UNESP] ; Mesquita, Luciane dos Reis [UNESP] ; Antunes, Carina Bueno ; Lainetti, Patrícia de Faria [UNESP] ; Fonseca-Alves, Carlos Eduardo [UNESP]
Date: 2021
Persistent ID: http://hdl.handle.net/11449/207381
Origin: Oasisbr
Subject(s): electron microscopy; fibrin membrane; histology; immunohistochemical; L-PRF; platelet; electron microscopy; electron microscopy; fibrin membrane; fibrin membrane; histology; histology; immunohistochemical; immunohistochemical; L-PRF; L-PRF; platelet; platelet
Description
Made available in DSpace on 2021-06-25T10:54:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
Autologous platelet concentrates have been used in regenerative medicine in humans due to the abundance of growth factors, but there are only a few reports in small animals. This study aimed to prepare and characterize a leukocyte and platelet-rich fibrin membrane (L-PRF) produced with blood obtained from cats. Thirteen client-owned healthy adult Maine Coon cats were enrolled. The blood samples were collected and centrifuged at 650g for 12 min using a centrifuge specifically designed for this application. The L-PRF clot was removed from the tube and red blood cell base layer was separated, leaving buffy coat intact. After this, L-PRF clot was compressed by specialized metal plate for 30–60 s, and L-PRF membrane was obtained. Light microscopy examination of the membranes showed three distinct layers: white part, buffy coat, and red part. Immunohistochemical analysis demonstrated expression of vascular endothelial growth factor and platelet derived growth factor. The scanning electron microscopy showed that three-dimensional architecture of fibrin network was more compact in the area near the buffy coat. In conclusion, the method used allowed the characterization of the L-PRF membrane composition, which presented cell types and fibrin network architecture similar to those described in the human species.
Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP)
Department of Dentistry FACOPH School—Natal
Department of Anatomy Institute of Biosciences—São Paulo State University (UNESP)
Department of Veterinary Medicine Anhanguera University
Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP)
Department of Anatomy Institute of Biosciences—São Paulo State University (UNESP)
