Description
Made available in DSpace on 2021-06-25T11:05:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Objectives: The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods: Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, ± 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results: GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion: PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. Clinical relevance: The PEMF therapy is an effective alternative method for optimizing bone healing.
Department of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos, Av Engenheiro Francisco José Longo, 777, São Dimas
Department of Bioscience and Bucal Diagnosis São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos
Department of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos, Av Engenheiro Francisco José Longo, 777, São Dimas
Department of Bioscience and Bucal Diagnosis São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos
CAPES: 001
FAPESP: 2018/13942-8
FAPESP: 2019/01669-8
CNPq: CNPq – 465259/2014-6
