Author(s):
Coscueta, Ezequiel R. ; Batista, Patrícia ; Gomes, José Erick Galindo [UNESP] ; da Silva, Roberto [UNESP] ; Pintado, Maria Manuela
Date: 2022
Persistent ID: http://hdl.handle.net/11449/223500
Origin: Oasisbr
Subject(s): ACE inhibition; Antihypertensive activity; Antioxidant activity; Bioactive peptide; Bioinformatics; In silico prediction; ORAC; ACE inhibition; ACE inhibition; Antihypertensive activity; Antihypertensive activity; Antioxidant activity; Antioxidant activity; Bioactive peptide; Bioactive peptide; Bioinformatics; Bioinformatics; In silico prediction; In silico prediction; ORAC; ORAC
Description
Made available in DSpace on 2022-04-28T19:50:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential.
Centro de Biotecnologia e Química Fina (CBQF) Laboratório Associado Escola Superior de Biotecnologia Universidade Católica Portuguesa, Rua Diogo Botelho 1327
Human Neurobehavioral Laboratory Research Centre for Human Development (HNL/CEDH) Universidade Católica Portuguesa, Rua Diogo Botelho 1327
Instituto de Biociências Letras e Ciências Exatas (IBILCE)-Rua Cristovão Colombo Campus São José do Rio Preto Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, SP
Laboratório de Microbiologia Tecnologia Enzimática e Bioprodutos—Universidade Federal do Agreste de Pernambuco (UFAPE), Avenida Bom Pastor s/n, Boa Vista, PE
Instituto de Biociências Letras e Ciências Exatas (IBILCE)-Rua Cristovão Colombo Campus São José do Rio Preto Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, SP
FAPESP: 2017/16482-5
CNPq: 202186/2018-0
