Author(s): Silva, Maria Emilia Bonifácio da [UNESP] ; Marin, José Moacir
Date: 2014
Persistent ID: http://hdl.handle.net/11449/26617
Origin: Oasisbr
Subject(s): Haemophilus influenzae; gene cap; creche; Haemophilus influenzae; cap gene; day-care center; Haemophilus influenzae; Haemophilus influenzae; gene cap; gene cap; creche; creche; Haemophilus influenzae; Haemophilus influenzae; cap gene; cap gene; day-care center; day-care center
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Cinqüenta e oito cepas de Haemophilus influenzae foram isoladas da nasofaringe de crianças saudáveis que freqüentam uma creche, e através da técnica de Southern blot foi pesquisada nas cepas acapsuladas a presença de seqüências do gene capsular. Sete cepas (12%) caracterizadas sorologicamente como acapsuladas mostraram homologia com seqüências específicas da cápsula. Uma cepa foi caracterizada com uma linhagem H. influenzae tipo b cápsula deficiente.
Fifty-eight nasopharyngeal isolates of Haemophilus influenzae were collected from healthy children at a day care center, and nontypeable isolates were examined by Southern blot for the presence of capsule gene sequences. Seven isolates (12%) demonstrated homology with capsule-specific sequences. One isolate was characterized as an H. influenzae type b capsule-deficient strain.
Universidade Estadual Paulista Departamento de Microbiologia
Universidade de São Paulo Faculdade de Odontologia Departamento de Morfologia, Estomatologia e Fisiologia
Universidade Estadual Paulista Departamento de Microbiologia
