Author(s):
Garrine, Marcelino ; Andrade, Mariana ; Neves, Joana ; Mandomando, Inácio ; Couto, Isabel ; Costa, Sofia Santos
Date: 2024
Persistent ID: http://hdl.handle.net/10362/169394
Origin: Repositório Institucional da UNL
Subject(s): Infectious Diseases; Pediatrics, Perinatology, and Child Health; Immunology and Microbiology(all); Microbiology (medical); SDG 3 - Good Health and Well-being; Infectious Diseases; Infectious Diseases; Pediatrics, Perinatology, and Child Health; Pediatrics, Perinatology, and Child Health; Immunology and Microbiology(all); Immunology and Microbiology(all); Microbiology (medical); Microbiology (medical); SDG 3 - Good Health and Well-being; SDG 3 - Good Health and Well-being
Description
Funding Information: This study was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia (FCT, Portugal) through funds to GHTM\u2014UID/04413/2020, LA-REAL\u2014LA/P/0117/2020 and to the Project DREBI, Ref. 2022.07931.PTDC ( https://doi.org/10.54499/2022.07931.PTDC ). CISM receives core funding from \u201CAgencia Espa\u00F1ola de Cooperacion Internacional para el Desarollo (AECID)\u201D. MG was supported by grant 145278, from Funda\u00E7\u00E3o Calouste Gulbenkian \u201CCalouste Gulbenkian Foundation, Portugal\u201D. MA was supported by grant UI/BD/154472/2022 and JN by grant 2022.07931.PTDC from FCT, Portugal. SSC was supported by FCT through CEECINST/00042/2021/CP1773/CT0009 ( https://doi.org/10.54499/CEECINST/00042/2021/CP1773/CT0009 ). Publisher Copyright: © The Author(s) 2024.
Staphylococcus aureus is a frequent agent of bacteraemia. This bacterium has a variety of virulence traits that allow the establishment and maintenance of infection. This study explored the virulence profile of S. aureus strains causing paediatric bacteraemia (SAB) in Manhiça district, Mozambique. We analysed 336 S. aureus strains isolated from blood cultures of children younger than 5 years admitted to the Manhiça District Hospital between 2001 and 2019, previously characterized for antibiotic susceptibility and clonality. The strains virulence potential was evaluated by PCR detection of the Panton-Valentine leucocidin (PVL) encoding genes, lukS-PV/lukF-PV, assessment of the capacity for biofilm formation and pathogenicity assays in Galleria mellonella. The overall carriage of PVL-encoding genes was over 40%, although reaching ~ 70 to 100% in the last years (2014 to 2019), potentially linked to the emergence of CC152 lineage. Strong biofilm production was a frequent trait of CC152 strains. Representative CC152 and CC121 strains showed higher virulence potential in the G. mellonella model when compared to reference strains, with variations within and between CCs. Our results highlight the importance of monitoring the emergent CC152-MSSA-PVL+ and other lineages, as they display important virulence traits that may negatively impact the management of SAB paediatric patients in Manhiça district, Mozambique.
