Author(s): Goulart,Antonio José ; Carmona,Eleonora Cano ; Monti,Rubens
Date: 2005
Origin: Oasisbr
Subject(s): alkaline xylanase; Rhizopus stolonifer; solid-state fermentation; alkaline xylanase; alkaline xylanase; Rhizopus stolonifer; Rhizopus stolonifer; solid-state fermentation; solid-state fermentation
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.
