Publicação
miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis
| Resumo: | The SnRK1 protein kinase, the plant ortholog of mammalian AMPK and yeast Snf1, is activated by the energy depletion caused by adverse environmental conditions. Upon activation, SnRK1 triggers extensive transcriptional changes to restore homeostasis and promote stress tolerance and survival partly through the inhibition of anabolism and the activation of catabolism. Despite the identification of a few bZIP transcription factors as downstream effectors, the mechanisms underlying gene regulation, and in particular gene repression by SnRK1, remain mostly unknown. microRNAs (miRNAs) are 20-24 nt RNAs that regulate gene expression post-transcriptionally by driving the cleavage and/or translation attenuation of complementary mRNA targets. In addition to their role in plant development, mounting evidence implicates miRNAs in the response to environmental stress. Given the involvement of miRNAs in stress responses and the fact that some of the SnRK1-regulated genes are miRNA targets, we postulated that miRNAs drive part of the transcriptional reprogramming triggered by SnRK1. By comparing the transcriptional response to energy deprivation between WT and dcl1-9, a mutant deficient in miRNA biogenesis, we identified 831 starvation genes misregulated in the dcl1-9 mutant, out of which 155 are validated or predicted miRNA targets. Functional clustering analysis revealed that the main cellular processes potentially co-regulated by SnRK1 and miRNAs are translation and organelle function and uncover TCP transcription factors as one of the most highly enriched functional clusters. TCP repression during energy deprivation was impaired in miR319 knockdown (MIM319) plants, demonstrating the involvement of miR319 in the stress-dependent regulation of TCPs. Altogether, our data indicates that miRNAs are components of the SnRK1 signaling cascade contributing to the regulation of specific mRNA targets and possibly tuning down particular cellular processes during the stress response. |
|---|---|
| Autores principais: | Confraria, Ana |
| Outros Autores: | Martinho, Cláudia; Elias, Alexandre; Rubio-Somoza, Ignacio; Baena-González, Elena |
| Assunto: | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| Idioma: | inglês |
| Origem: | ARCA |
| _version_ | 1867149334270705664 |
|---|---|
| author | Confraria, Ana |
| author2 | Martinho, Cláudia Elias, Alexandre Rubio-Somoza, Ignacio Baena-González, Elena |
| author2_role | author author author author |
| author_facet | Confraria, Ana Martinho, Cláudia Elias, Alexandre Rubio-Somoza, Ignacio Baena-González, Elena |
| author_role | author |
| contributor_name_str_mv | ARCA |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Confraria, Ana\"},{\"Person.name\":\"Martinho, Cláudia\"},{\"Person.name\":\"Elias, Alexandre\"},{\"Person.name\":\"Rubio-Somoza, Ignacio\"},{\"Person.name\":\"Baena-González, Elena\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | ARCA |
| datacite.creators.creator.creatorName.fl_str_mv | Confraria, Ana Martinho, Cláudia Elias, Alexandre Rubio-Somoza, Ignacio Baena-González, Elena |
| datacite.date.Accepted.fl_str_mv | 2013-06-20T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-10-26T13:02:06Z |
| datacite.date.embargoed.fl_str_mv | 2015-10-26T13:02:06Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| datacite.titles.title.fl_str_mv | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| dc.contributor.none.fl_str_mv | ARCA |
| dc.creator.none.fl_str_mv | Confraria, Ana Martinho, Cláudia Elias, Alexandre Rubio-Somoza, Ignacio Baena-González, Elena |
| dc.date.Accepted.fl_str_mv | 2013-06-20T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-10-26T13:02:06Z |
| dc.date.embargoed.fl_str_mv | 2015-10-26T13:02:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.7/442 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers Research Foundation |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| dc.title.fl_str_mv | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The SnRK1 protein kinase, the plant ortholog of mammalian AMPK and yeast Snf1, is activated by the energy depletion caused by adverse environmental conditions. Upon activation, SnRK1 triggers extensive transcriptional changes to restore homeostasis and promote stress tolerance and survival partly through the inhibition of anabolism and the activation of catabolism. Despite the identification of a few bZIP transcription factors as downstream effectors, the mechanisms underlying gene regulation, and in particular gene repression by SnRK1, remain mostly unknown. microRNAs (miRNAs) are 20-24 nt RNAs that regulate gene expression post-transcriptionally by driving the cleavage and/or translation attenuation of complementary mRNA targets. In addition to their role in plant development, mounting evidence implicates miRNAs in the response to environmental stress. Given the involvement of miRNAs in stress responses and the fact that some of the SnRK1-regulated genes are miRNA targets, we postulated that miRNAs drive part of the transcriptional reprogramming triggered by SnRK1. By comparing the transcriptional response to energy deprivation between WT and dcl1-9, a mutant deficient in miRNA biogenesis, we identified 831 starvation genes misregulated in the dcl1-9 mutant, out of which 155 are validated or predicted miRNA targets. Functional clustering analysis revealed that the main cellular processes potentially co-regulated by SnRK1 and miRNAs are translation and organelle function and uncover TCP transcription factors as one of the most highly enriched functional clusters. TCP repression during energy deprivation was impaired in miR319 knockdown (MIM319) plants, demonstrating the involvement of miR319 in the stress-dependent regulation of TCPs. Altogether, our data indicates that miRNAs are components of the SnRK1 signaling cascade contributing to the regulation of specific mRNA targets and possibly tuning down particular cellular processes during the stress response. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://arca.gimm.pt/bitstreams/16f3bc86-835d-4cec-9cd2-1eddabc749a8/download |
| id | arca_6cdc23db19fd2aa4d136dbaa41699bfc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.7/442 |
| instacron_str | arca |
| institution | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| instname_str | Repositório do GIMM – Gulbenkian Institute for Molecular Medicine |
| language | eng |
| network_acronym_str | arca |
| network_name_str | ARCA |
| oai_identifier_str | oai:arca.gimm.pt:10400.7/442 |
| organization_str_mv | urn:organizationAcronym:arca |
| person_str_mv | Confraria, Ana Martinho, Cláudia Elias, Alexandre Rubio-Somoza, Ignacio Baena-González, Elena |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Frontiers Research Foundation |
| reponame_str | ARCA |
| repository_id_str | urn:repositoryAcronym:arca |
| service_str_mv | urn:repositoryAcronym:arca |
| spelling | engFrontiers Research Foundationpt_PTThe SnRK1 protein kinase, the plant ortholog of mammalian AMPK and yeast Snf1, is activated by the energy depletion caused by adverse environmental conditions. Upon activation, SnRK1 triggers extensive transcriptional changes to restore homeostasis and promote stress tolerance and survival partly through the inhibition of anabolism and the activation of catabolism. Despite the identification of a few bZIP transcription factors as downstream effectors, the mechanisms underlying gene regulation, and in particular gene repression by SnRK1, remain mostly unknown. microRNAs (miRNAs) are 20-24 nt RNAs that regulate gene expression post-transcriptionally by driving the cleavage and/or translation attenuation of complementary mRNA targets. In addition to their role in plant development, mounting evidence implicates miRNAs in the response to environmental stress. Given the involvement of miRNAs in stress responses and the fact that some of the SnRK1-regulated genes are miRNA targets, we postulated that miRNAs drive part of the transcriptional reprogramming triggered by SnRK1. By comparing the transcriptional response to energy deprivation between WT and dcl1-9, a mutant deficient in miRNA biogenesis, we identified 831 starvation genes misregulated in the dcl1-9 mutant, out of which 155 are validated or predicted miRNA targets. Functional clustering analysis revealed that the main cellular processes potentially co-regulated by SnRK1 and miRNAs are translation and organelle function and uncover TCP transcription factors as one of the most highly enriched functional clusters. TCP repression during energy deprivation was impaired in miR319 knockdown (MIM319) plants, demonstrating the involvement of miR319 in the stress-dependent regulation of TCPs. Altogether, our data indicates that miRNAs are components of the SnRK1 signaling cascade contributing to the regulation of specific mRNA targets and possibly tuning down particular cellular processes during the stress response.application/pdfpt_PTmiRNAs mediate SnRK1-dependent energy signaling in ArabidopsisConfraria, AnaMartinho, CláudiaElias, AlexandreRubio-Somoza, IgnacioBaena-González, ElenaHostingInstitutionOrganizationalARCAe-mailmailto:arca.repository@gimm.ptarca.repository@gimm.ptDOIIsPartOf10.3389/fpls.2013.001972015-10-26T13:02:06Z2013-06-202013-06-20T00:00:00ZHandlehttp://hdl.handle.net/10400.7/442http://purl.org/coar/access_right/c_abf2open accessArabidopsisSnRK1stressmiRNAenergy signalingDCL15464948 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2013-06-20http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://arca.gimm.pt/bitstreams/16f3bc86-835d-4cec-9cd2-1eddabc749a8/downloadFrontiers in Plant Science4121 |
| spellingShingle | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis Confraria, Ana Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| status | SINGLETON |
| subject.fl_str_mv | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| title | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| title_full | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| title_fullStr | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| title_full_unstemmed | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| title_short | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| title_sort | miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis |
| topic | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| topic_facet | Arabidopsis SnRK1 stress miRNA energy signaling DCL1 |
| url | http://hdl.handle.net/10400.7/442 |
| visible | 1 |