Publicação
Design and synthesis of molecularly imprinted polymers to target olive leaf secoiridoids
| Resumo: | The purification and concentration of bioactive compounds have become more relevant over time, as these compounds have applicability both at laboratory and industrial scale. Despite their importance, our knowledge of techniques to efficiently isolate these bioactive compounds is impecunious. At this juncture, molecularly imprinted polymeric materials (MIPs) capable of selective recognition and capture of secoiridoids present in olive leaves were synthesized. The combination of different monomers and polymerisation techniques has given rise to twenty-one molecularly imprinted materials comprising different morphologies. These materials were characterized and applied as adsorbents in solid phase extraction (SPE) cartridges, tests performed also included the commercial resins REILLEX402, REILLEX425, XAD4, XAD7HP, and DAX8, where preliminary adsorption studies corroborated the reported high-performance of materials containing the functional monomer 4-vinylpyridine (4VP) in both organic solvents and hydroalcoholic mixtures. In addition to oleuropein, tyrosol, vanillin, quercetin, verbascoside, vanillic acid, and caffeic acid were also considered as template molecules, as they tend to appear in olive leaf extracts. By using an olive leaf extract in adsorption/desorption cycles, fractionation and concentration of phenolic compounds present in the initial extract were achieved, thus luteolin-enriched fractions were obtained. The obtained results indicate that although different MIPs have been successfully synthesized, for the desired purpose the adsorbents MIP3 and MIP7 are the most adequate and they can be used in sequence with Reillex402 to optimize the purification/concentration process of the compounds present in the extracts. |
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| Autores principais: | Almeida, Ayssata Patrícia da Costa Leocádia Vaz |
| Assunto: | Molecular imprinting Polyphenols Secoiridoids Olive leaves extracts Fractionation Bio-circular economy |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | The purification and concentration of bioactive compounds have become more relevant over time, as these compounds have applicability both at laboratory and industrial scale. Despite their importance, our knowledge of techniques to efficiently isolate these bioactive compounds is impecunious. At this juncture, molecularly imprinted polymeric materials (MIPs) capable of selective recognition and capture of secoiridoids present in olive leaves were synthesized. The combination of different monomers and polymerisation techniques has given rise to twenty-one molecularly imprinted materials comprising different morphologies. These materials were characterized and applied as adsorbents in solid phase extraction (SPE) cartridges, tests performed also included the commercial resins REILLEX402, REILLEX425, XAD4, XAD7HP, and DAX8, where preliminary adsorption studies corroborated the reported high-performance of materials containing the functional monomer 4-vinylpyridine (4VP) in both organic solvents and hydroalcoholic mixtures. In addition to oleuropein, tyrosol, vanillin, quercetin, verbascoside, vanillic acid, and caffeic acid were also considered as template molecules, as they tend to appear in olive leaf extracts. By using an olive leaf extract in adsorption/desorption cycles, fractionation and concentration of phenolic compounds present in the initial extract were achieved, thus luteolin-enriched fractions were obtained. The obtained results indicate that although different MIPs have been successfully synthesized, for the desired purpose the adsorbents MIP3 and MIP7 are the most adequate and they can be used in sequence with Reillex402 to optimize the purification/concentration process of the compounds present in the extracts. |
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