Publicação
Application of apple cider residue on citric acid bioprocesses using Aspergillus niger under solid state fermentation
| Resumo: | Apple pomace (AP) is considered among the most damaging agro-industrial wastes to the environment as well as the economy. Therefore, lucrative alternatives have been studied to render this low-value waste into citric acid (CA), which is a high-value product that can be used in several fields such as pharmaceutical, cosmetics, agriculture and biochemical industries. Recently, apple cider residue (ACR) has also become an environmental hazard which, contrary to AP, has not yet been studied for its potential application in industrial bioprocesses. Within this context, the present study was carried out to evaluate the potential of ACR to produce CA through solid state fermentation (SSF) by different Aspergillus niger strains. For this, several natural A. niger strains and several variations of the fermentation substrates (AP, ACR, mixtures of AP+ACR, supplemented and not supplemented) were tested. Among the tested strains, MUM 92.13 proved to be the best strain for CA production using AP (41.61 ± 1.14 g/kg), while with ACR the production of CA was lower (13.99 ± 0.09 g/kg). On the other hand, high glycerol production (46 ±0.89 g/kg) and yield (0.92 ±0.08 g/g total sugars) was obtained with ACR when supplements were not added. When both matrices were mixed (75%AP+25%ACR and 50%AP+50%ACR), CA yield of supplemented 75% AP+25%ACR was similar to that obtained with AP, but with lower production (24.81 ±1.24 g/kg). Higher glycerol values of 54.81 ± 0.44 g/kg were achieved with non-supplemented 50%AP+50%ACR. Non supplemented ACR achieved a promising glycerol yield of 2.19 ±0.49 g/g total sugars. ACR thus proved to be a better substrate for glycerol production than for CA, when compared with AP, and can serve as a natural substrate for SSF, which is an economical alternative technique and does not require sophisticated instruments and techniques. |
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| Autores principais: | Ben Salha, Asma |
| Assunto: | Glycerol Apple pomace Agro-industrial wastes Biotechnology Circular economy |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Apple pomace (AP) is considered among the most damaging agro-industrial wastes to the environment as well as the economy. Therefore, lucrative alternatives have been studied to render this low-value waste into citric acid (CA), which is a high-value product that can be used in several fields such as pharmaceutical, cosmetics, agriculture and biochemical industries. Recently, apple cider residue (ACR) has also become an environmental hazard which, contrary to AP, has not yet been studied for its potential application in industrial bioprocesses. Within this context, the present study was carried out to evaluate the potential of ACR to produce CA through solid state fermentation (SSF) by different Aspergillus niger strains. For this, several natural A. niger strains and several variations of the fermentation substrates (AP, ACR, mixtures of AP+ACR, supplemented and not supplemented) were tested. Among the tested strains, MUM 92.13 proved to be the best strain for CA production using AP (41.61 ± 1.14 g/kg), while with ACR the production of CA was lower (13.99 ± 0.09 g/kg). On the other hand, high glycerol production (46 ±0.89 g/kg) and yield (0.92 ±0.08 g/g total sugars) was obtained with ACR when supplements were not added. When both matrices were mixed (75%AP+25%ACR and 50%AP+50%ACR), CA yield of supplemented 75% AP+25%ACR was similar to that obtained with AP, but with lower production (24.81 ±1.24 g/kg). Higher glycerol values of 54.81 ± 0.44 g/kg were achieved with non-supplemented 50%AP+50%ACR. Non supplemented ACR achieved a promising glycerol yield of 2.19 ±0.49 g/g total sugars. ACR thus proved to be a better substrate for glycerol production than for CA, when compared with AP, and can serve as a natural substrate for SSF, which is an economical alternative technique and does not require sophisticated instruments and techniques. |
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