Publicação
Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke
| Resumo: | Occupational exposure as a firefighter is classified as carcinogenic to humans. However, information on the biological effects of wildland firefighting remains limited. This study aimed to assess genotoxicity in a group of wildland firefighters and evaluate the contribution of total concentration of urinary hydroxylated polycyclic aromatic hydrocarbons (ΣOHPAHs) to selected endpoints. A group of 59 northern Portuguese wildland firefighters (mean age: 35.5 ± 9.0 years) was evaluated before and during the wildfire season. Sociodemographic, lifestyle, occupational-related information was collected via questionnaire. The micronucleus (MN) assay in buccal and urothelial cells was applied to paired samples to assess the association with exposure, as indicated by urinary ΣOHPAHs levels. The risk of MN formation in urothelial cells was over twofold higher during the wildfire season [Frequency Ratio (FR): 2.13, 95 % CI: 1.99–2.27, p = 0.01]. A 35 % increase in MN frequency in buccal cells was observed during the wildfire season (FR: 1.35, 95 % CI: 0.76–2.40, p > 0.05). Urinary ΣOHPAHs exhibited a significant positive association with MN‰ in urothelial cells (FR:1.04, 95 % CI: 1.01–1.08, p < 0.05). This study provides the first assessment of MN frequency in urothelial cells of wildland firefighters, offering novel evidence of genotoxic risks and potential long-term health impacts associated with wildland firefighting. |
|---|---|
| Autores principais: | Esteves, Filipa |
| Outros Autores: | Madureira, Joana; Barros, Bela; Alves, Sara; Vaz, Josiana A.; Oliveira, Marta; Slezakova, Klara; Fernandes, Adília; Pereira, Maria do Carmo; Morais, Simone; Bonassi, Stefano; Teixeira, João P.; Costa, Solange |
| Assunto: | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173417093955584 |
|---|---|
| author | Esteves, Filipa |
| author2 | Madureira, Joana Barros, Bela Alves, Sara Vaz, Josiana A. Oliveira, Marta Slezakova, Klara Fernandes, Adília Pereira, Maria do Carmo Morais, Simone Bonassi, Stefano Teixeira, João P. Costa, Solange |
| author2_role | author author author author author author author author author author author author |
| author_facet | Esteves, Filipa Madureira, Joana Barros, Bela Alves, Sara Vaz, Josiana A. Oliveira, Marta Slezakova, Klara Fernandes, Adília Pereira, Maria do Carmo Morais, Simone Bonassi, Stefano Teixeira, João P. Costa, Solange |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Esteves, Filipa\"},{\"Person.name\":\"Madureira, Joana\"},{\"Person.name\":\"Barros, Bela\"},{\"Person.name\":\"Alves, Sara\"},{\"Person.name\":\"Vaz, Josiana A.\",\"Person.identifier.orcid\":\"0000-0002-6989-1169\"},{\"Person.name\":\"Oliveira, Marta\"},{\"Person.name\":\"Slezakova, Klara\"},{\"Person.name\":\"Fernandes, Adília\",\"Person.identifier.orcid\":\"0000-0003-1658-4509\"},{\"Person.name\":\"Pereira, Maria do Carmo\"},{\"Person.name\":\"Morais, Simone\"},{\"Person.name\":\"Bonassi, Stefano\"},{\"Person.name\":\"Teixeira, João P.\"},{\"Person.name\":\"Costa, Solange\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Esteves, Filipa Madureira, Joana Barros, Bela Alves, Sara Vaz, Josiana A. Oliveira, Marta Slezakova, Klara Fernandes, Adília Pereira, Maria do Carmo Morais, Simone Bonassi, Stefano Teixeira, João P. Costa, Solange |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-03-23T15:04:01Z |
| datacite.date.embargoed.fl_str_mv | 2026-03-23T15:04:01Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| datacite.titles.title.fl_str_mv | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Esteves, Filipa Madureira, Joana Barros, Bela Alves, Sara Vaz, Josiana A. Oliveira, Marta Slezakova, Klara Fernandes, Adília Pereira, Maria do Carmo Morais, Simone Bonassi, Stefano Teixeira, João P. Costa, Solange |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-03-23T15:04:01Z |
| dc.date.embargoed.fl_str_mv | 2026-03-23T15:04:01Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/36224 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| dc.title.fl_str_mv | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Occupational exposure as a firefighter is classified as carcinogenic to humans. However, information on the biological effects of wildland firefighting remains limited. This study aimed to assess genotoxicity in a group of wildland firefighters and evaluate the contribution of total concentration of urinary hydroxylated polycyclic aromatic hydrocarbons (ΣOHPAHs) to selected endpoints. A group of 59 northern Portuguese wildland firefighters (mean age: 35.5 ± 9.0 years) was evaluated before and during the wildfire season. Sociodemographic, lifestyle, occupational-related information was collected via questionnaire. The micronucleus (MN) assay in buccal and urothelial cells was applied to paired samples to assess the association with exposure, as indicated by urinary ΣOHPAHs levels. The risk of MN formation in urothelial cells was over twofold higher during the wildfire season [Frequency Ratio (FR): 2.13, 95 % CI: 1.99–2.27, p = 0.01]. A 35 % increase in MN frequency in buccal cells was observed during the wildfire season (FR: 1.35, 95 % CI: 0.76–2.40, p > 0.05). Urinary ΣOHPAHs exhibited a significant positive association with MN‰ in urothelial cells (FR:1.04, 95 % CI: 1.01–1.08, p < 0.05). This study provides the first assessment of MN frequency in urothelial cells of wildland firefighters, offering novel evidence of genotoxic risks and potential long-term health impacts associated with wildland firefighting. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/0b92250b-dc71-43ea-adce-f439b611692d/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | PCIF/SSO/0017/2018 UIDB/04750/2020 LA/P/0064/2020 |
| funding.name_str_mv | 3599-PPCDT 6817 - DCRRNI ID 6817 - DCRRNI ID |
| funding_str_mv | PCIF/SSO/0017/2018 info:eu-repo/grantAgreement/FCT/3599-PPCDT/PCIF%2FSSO%2F0017%2F2018/PT UIDB/04750/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT LA/P/0064/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PT |
| id | ipb_bf998c2cfd8d463c28c6f1f4a41cdfca |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/36224 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/36224 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Esteves, Filipa Madureira, Joana Barros, Bela Alves, Sara Vaz, Josiana A. Vaz, Josiana A. https://www.ciencia-id.pt/171B-88BA-64F5 171B-88BA-64F5 http://orcid.org/0000-0002-6989-1169 0000-0002-6989-1169 Oliveira, Marta Slezakova, Klara Fernandes, Adília Fernandes, Adília https://www.ciencia-id.pt/0514-76CD-8CCF 0514-76CD-8CCF http://orcid.org/0000-0003-1658-4509 0000-0003-1658-4509 Pereira, Maria do Carmo Morais, Simone Bonassi, Stefano Teixeira, João P. Costa, Solange |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engElsevierengOccupational exposure as a firefighter is classified as carcinogenic to humans. However, information on the biological effects of wildland firefighting remains limited. This study aimed to assess genotoxicity in a group of wildland firefighters and evaluate the contribution of total concentration of urinary hydroxylated polycyclic aromatic hydrocarbons (ΣOHPAHs) to selected endpoints. A group of 59 northern Portuguese wildland firefighters (mean age: 35.5 ± 9.0 years) was evaluated before and during the wildfire season. Sociodemographic, lifestyle, occupational-related information was collected via questionnaire. The micronucleus (MN) assay in buccal and urothelial cells was applied to paired samples to assess the association with exposure, as indicated by urinary ΣOHPAHs levels. The risk of MN formation in urothelial cells was over twofold higher during the wildfire season [Frequency Ratio (FR): 2.13, 95 % CI: 1.99–2.27, p = 0.01]. A 35 % increase in MN frequency in buccal cells was observed during the wildfire season (FR: 1.35, 95 % CI: 0.76–2.40, p > 0.05). Urinary ΣOHPAHs exhibited a significant positive association with MN‰ in urothelial cells (FR:1.04, 95 % CI: 1.01–1.08, p < 0.05). This study provides the first assessment of MN frequency in urothelial cells of wildland firefighters, offering novel evidence of genotoxic risks and potential long-term health impacts associated with wildland firefighting.application/pdfengMicronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smokeEsteves, FilipaMadureira, JoanaBarros, BelaAlves, SaraPersonalVaz, Josiana A.DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18VazJosiana A.Ciência IDhttps://www.ciencia-id.pt171B-88BA-64F5ORCIDhttp://orcid.org0000-0002-6989-1169Scopus Author IDhttps://www.scopus.com16305323200Oliveira, MartaSlezakova, KlaraPersonalFernandes, AdíliaDSpacehttp://dspace.org/items/bdfceda8-18d6-4b63-b6f9-d3870cd0dbb0DSpacehttp://dspace.org/items/bdfceda8-18d6-4b63-b6f9-d3870cd0dbb0FernandesAdíliaCiência IDhttps://www.ciencia-id.pt0514-76CD-8CCFORCIDhttp://orcid.org0000-0003-1658-4509Researcher IDhttps://www.researcherid.comV-8399-2017Scopus Author IDhttps://www.scopus.com56489572200Pereira, Maria do CarmoMorais, SimoneBonassi, StefanoTeixeira, João P.Costa, SolangeHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0304-3894DOIIsPartOf10.1016/j.jhazmat.2025.1404272026-03-23T15:04:01Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/36224http://purl.org/coar/access_right/c_abf2open accessChromosomal damageDNA damageMicronucleus assayWildfire smokeWildland firefighters1308640 bytesFundação para a Ciência e a TecnologiaBioFirEx: Um painel de (bio)marcadores para a vigilância da saúde e da segurança do bombeiroinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PCIF%2FSSO%2F0017%2F2018/PTPCIF/SSO/0017/20183599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaEpidemiology Research Unit - Institute of Public Health, University of Portoinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PTUIDB/04750/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaLaboratory for Integrative and Translational Research in Population Healthinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PTLA/P/0064/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/0b92250b-dc71-43ea-adce-f439b611692d/downloadJournal of Hazardous Materials500 |
| spellingShingle | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke Esteves, Filipa Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| status | SINGLETON |
| subject.fl_str_mv | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| title | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| title_full | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| title_fullStr | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| title_full_unstemmed | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| title_short | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| title_sort | Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke |
| topic | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| topic_facet | Chromosomal damage DNA damage Micronucleus assay Wildfire smoke Wildland firefighters |
| url | http://hdl.handle.net/10198/36224 |
| visible | 1 |
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