Publicação
Candida albicans enhanced phospholipase production after exposition to a static non-uniform magnetic field
| Resumo: | INTRODUCTION: Microbial virulence factors are responsible for tissue damage in hosts. Candida albicans is an opportunistic pathogen that constitutes an increasing risk of infection, especially for immunosuppressed or immunocompromised patients. OBJECTIVE: The objective of this study was to determine the effect of a static non-uniform magnetic field on the phenotype expression of different strains of Candida albicans. METHODS: The strains of Candida albicans were grown on phospholipase-agar, according to Shimizu et al. (1996) and incubated at 37 ºC inside a magnetic field (except the assays used as blank). The magnetic field was generated by two magnetite plates (Figure 1) and standardized as a function of distance versus number of magnetic plates (Figure 2). The magnetic field was of 500 gauss in the central part between the two magnetic plates. RESULTS: The preliminary results show a visible increase in the halo formed due to phospholipase production, suggesting that the exposition to a magnetic field can enhance the expression of this virulence factor. |
|---|---|
| Autores principais: | Gasparetto, A. |
| Outros Autores: | Svidzinsky, T. E.; Paula, Claudete Rodrigues; Oliveira, Rosário; Azeredo, Joana |
| Ano: | 2002 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | INTRODUCTION: Microbial virulence factors are responsible for tissue damage in hosts. Candida albicans is an opportunistic pathogen that constitutes an increasing risk of infection, especially for immunosuppressed or immunocompromised patients. OBJECTIVE: The objective of this study was to determine the effect of a static non-uniform magnetic field on the phenotype expression of different strains of Candida albicans. METHODS: The strains of Candida albicans were grown on phospholipase-agar, according to Shimizu et al. (1996) and incubated at 37 ºC inside a magnetic field (except the assays used as blank). The magnetic field was generated by two magnetite plates (Figure 1) and standardized as a function of distance versus number of magnetic plates (Figure 2). The magnetic field was of 500 gauss in the central part between the two magnetic plates. RESULTS: The preliminary results show a visible increase in the halo formed due to phospholipase production, suggesting that the exposition to a magnetic field can enhance the expression of this virulence factor. |
|---|