Publicação
Interactions between glycerol, PEG-200 and (NH4)2 SO4 in the stability of heterologous cutinase
| Resumo: | Cutinases (EC 3.1.1.74)are versatile enzymes that have hydrolytic activity on various esters [1]. The spacial structure and the catalytic site of the enzymes can be protected by chemical additives to promove the stability of the activity [2, 3]. The goal of this work was to improve the stability of a recombinant cutinase produced by Escherichia coli. |
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| Autores principais: | Gomes, Daniela S. |
| Outros Autores: | Matamá, Maria Teresa; Paulo, Artur Cavaco; Jordão, R. C. C.; Takaki, G. M. Campos; Salgueiro, Alexandra A. |
| Assunto: | Heterologous cutinase Enzyme stabilization |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Cutinases (EC 3.1.1.74)are versatile enzymes that have hydrolytic activity on various esters [1]. The spacial structure and the catalytic site of the enzymes can be protected by chemical additives to promove the stability of the activity [2, 3]. The goal of this work was to improve the stability of a recombinant cutinase produced by Escherichia coli. |
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