Publicação
Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity
| Resumo: | Introduction: Mixed bacterial-fungal colonization of the endotracheal tubes is now evident, with microbial interplay withstanding common antimicrobial therapy and paying for persistent and severe VAP infections. While alternative therapeutic strategies effectively targeting inter-kingdom biofilms are required, the role of each microorganism need to be appraised to deliver effective treatments. Hypothesis and aims: We earlier reported the combination therapy involving polymyxin B (PMB) and amphotericin B (AMB) as holding an attractive therapeutic option to treat dual-species biofilms. This study aimed to determine the post-antimicrobial phenomenon of PMB/AMB combined action in P. aeruginosa (PA) +Candida albicans (CA) biofilms, and to ascertain the events underlying biofilm growth restoration. Methodology: Post-antimicrobial effect of PMB combined with AMB was assessed in 24-h dual-species biofilms. Cell culturability and viability were evaluated by CFU and Live/Dead staining, respectively. The gene expression profile was assessed by qPCR. Results: Results showed that PA+CA biofilms lost their culturability straightaway being exposed to PMB/AMB combined solution. However, 24h was enough to both species recover their growth onto agar medium, with microbial counts approximating those observed for pre-treated biofilms. Following the subsequent treatment cycle, CFU estimation was only slightly disturbed. L/D results revealed that PA and CA populations displayed a compromised status at the end of the first PMB/AMB treatment cycle. Finishing the 24-h-regrowth cycle, most biofilm-encased species exhibited viability, which endured after the second treatment period. Transcriptional analysis of dual-species biofilms exposed to PMB/AMB combined action showed a high expression level in all PA resistance-encoded genes anrB, galU, mexA and algD and in ERG3 and ALS2 CA genes. Conclusion: Our finsings showed that PA+CA biofilms were able to escape to the combined action of PMB/AMB, and both species had a preeminent role while retaining adaptive resistance mechanisms that likely contributed for their recovery and adaptation on the ensuing treatments. |
|---|---|
| Autores principais: | Grainha, Tânia |
| Outros Autores: | Lopes, Susana Patrícia; Rodrigues, Maria Elisa Costa; Magalhães, Andreia Patrícia Alves; Pereira, Maria Olívia |
| Assunto: | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439098461945856 |
|---|---|
| author | Grainha, Tânia |
| author2 | Lopes, Susana Patrícia Rodrigues, Maria Elisa Costa Magalhães, Andreia Patrícia Alves Pereira, Maria Olívia |
| author2_role | author author author author |
| author_facet | Grainha, Tânia Lopes, Susana Patrícia Rodrigues, Maria Elisa Costa Magalhães, Andreia Patrícia Alves Pereira, Maria Olívia |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Grainha, Tânia\"},{\"Person.name\":\"Lopes, Susana Patrícia\"},{\"Person.name\":\"Rodrigues, Maria Elisa Costa\"},{\"Person.name\":\"Magalhães, Andreia Patrícia Alves\"},{\"Person.name\":\"Pereira, Maria Olívia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Grainha, Tânia Lopes, Susana Patrícia Rodrigues, Maria Elisa Costa Magalhães, Andreia Patrícia Alves Pereira, Maria Olívia |
| datacite.date.Accepted.fl_str_mv | 2019-09-03T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-10-11T16:36:35Z |
| datacite.date.embargoed.fl_str_mv | 2019-10-11T16:36:35Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| datacite.titles.title.fl_str_mv | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Grainha, Tânia Lopes, Susana Patrícia Rodrigues, Maria Elisa Costa Magalhães, Andreia Patrícia Alves Pereira, Maria Olívia |
| dc.date.Accepted.fl_str_mv | 2019-09-03T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-10-11T16:36:35Z |
| dc.date.embargoed.fl_str_mv | 2019-10-11T16:36:35Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/61756 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| dc.title.fl_str_mv | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | Introduction: Mixed bacterial-fungal colonization of the endotracheal tubes is now evident, with microbial interplay withstanding common antimicrobial therapy and paying for persistent and severe VAP infections. While alternative therapeutic strategies effectively targeting inter-kingdom biofilms are required, the role of each microorganism need to be appraised to deliver effective treatments. Hypothesis and aims: We earlier reported the combination therapy involving polymyxin B (PMB) and amphotericin B (AMB) as holding an attractive therapeutic option to treat dual-species biofilms. This study aimed to determine the post-antimicrobial phenomenon of PMB/AMB combined action in P. aeruginosa (PA) +Candida albicans (CA) biofilms, and to ascertain the events underlying biofilm growth restoration. Methodology: Post-antimicrobial effect of PMB combined with AMB was assessed in 24-h dual-species biofilms. Cell culturability and viability were evaluated by CFU and Live/Dead staining, respectively. The gene expression profile was assessed by qPCR. Results: Results showed that PA+CA biofilms lost their culturability straightaway being exposed to PMB/AMB combined solution. However, 24h was enough to both species recover their growth onto agar medium, with microbial counts approximating those observed for pre-treated biofilms. Following the subsequent treatment cycle, CFU estimation was only slightly disturbed. L/D results revealed that PA and CA populations displayed a compromised status at the end of the first PMB/AMB treatment cycle. Finishing the 24-h-regrowth cycle, most biofilm-encased species exhibited viability, which endured after the second treatment period. Transcriptional analysis of dual-species biofilms exposed to PMB/AMB combined action showed a high expression level in all PA resistance-encoded genes anrB, galU, mexA and algD and in ERG3 and ALS2 CA genes. Conclusion: Our finsings showed that PA+CA biofilms were able to escape to the combined action of PMB/AMB, and both species had a preeminent role while retaining adaptive resistance mechanisms that likely contributed for their recovery and adaptation on the ensuing treatments. |
| dirty | 0 |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/61756 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/61756 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Grainha, Tânia Lopes, Susana Patrícia Rodrigues, Maria Elisa Costa Magalhães, Andreia Patrícia Alves Pereira, Maria Olívia |
| publishDate | 2019 |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engporIntroduction: Mixed bacterial-fungal colonization of the endotracheal tubes is now evident, with microbial interplay withstanding common antimicrobial therapy and paying for persistent and severe VAP infections. While alternative therapeutic strategies effectively targeting inter-kingdom biofilms are required, the role of each microorganism need to be appraised to deliver effective treatments. Hypothesis and aims: We earlier reported the combination therapy involving polymyxin B (PMB) and amphotericin B (AMB) as holding an attractive therapeutic option to treat dual-species biofilms. This study aimed to determine the post-antimicrobial phenomenon of PMB/AMB combined action in P. aeruginosa (PA) +Candida albicans (CA) biofilms, and to ascertain the events underlying biofilm growth restoration. Methodology: Post-antimicrobial effect of PMB combined with AMB was assessed in 24-h dual-species biofilms. Cell culturability and viability were evaluated by CFU and Live/Dead staining, respectively. The gene expression profile was assessed by qPCR. Results: Results showed that PA+CA biofilms lost their culturability straightaway being exposed to PMB/AMB combined solution. However, 24h was enough to both species recover their growth onto agar medium, with microbial counts approximating those observed for pre-treated biofilms. Following the subsequent treatment cycle, CFU estimation was only slightly disturbed. L/D results revealed that PA and CA populations displayed a compromised status at the end of the first PMB/AMB treatment cycle. Finishing the 24-h-regrowth cycle, most biofilm-encased species exhibited viability, which endured after the second treatment period. Transcriptional analysis of dual-species biofilms exposed to PMB/AMB combined action showed a high expression level in all PA resistance-encoded genes anrB, galU, mexA and algD and in ERG3 and ALS2 CA genes. Conclusion: Our finsings showed that PA+CA biofilms were able to escape to the combined action of PMB/AMB, and both species had a preeminent role while retaining adaptive resistance mechanisms that likely contributed for their recovery and adaptation on the ensuing treatments.application/pdfapplication/pdfporPseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activityGrainha, TâniaLopes, Susana PatríciaRodrigues, Maria Elisa CostaMagalhães, Andreia Patrícia AlvesPereira, Maria OlíviaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONGrainha, Tânia; Lopes, Susana P.; Rodrigues, M. Elisa; Magalhães, Andreia P.; Pereira, Maria Olívia, Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity. EUROBIOFILMS 2019 - 6th European Congress on Biofilms. No. 164, Glasgow, Scotland, Sep 3-6, 181, 2019.2019-10-11T16:36:35Z2019-09-032019-10-11T13:36:10Z2019-09-03T00:00:00ZHandlehttps://hdl.handle.net/1822/61756http://purl.org/coar/access_right/c_abf2open accesshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Médicas::Biotecnologia Médicahttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Engenharia e Tecnologia::Biotecnologia Industrial95227 bytes220279 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/8ebea5d6-4969-4027-a099-caea8e10db3e/downloadhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/f0cbeda8-b72b-4282-b461-5dd21a0f3e0b/download |
| spellingShingle | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity Grainha, Tânia Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| status | SINGLETON |
| subject.other.fl_str_mv | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| title | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| title_full | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| title_fullStr | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| title_full_unstemmed | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| title_short | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| title_sort | Pseudomonas aeruginosa-Candida albicans polymicrobial biofilms in ventilator-associated infections (VAP): evaluating the post-antimicrobial effect of amphotericin B/polymyxin B combined activity |
| topic | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| topic_facet | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial |
| url | https://hdl.handle.net/1822/61756 |
| visible | 1 |