Publicação
Improvement of the slide culture technique for the assessment of yeast viability
| Resumo: | This work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry. |
|---|---|
| Autores principais: | Cruyt, Frederik |
| Outros Autores: | Sousa, C. A.; Machado, Manuela D.; Soares, Eduardo V. |
| Assunto: | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438482474926080 |
|---|---|
| author | Cruyt, Frederik |
| author2 | Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
| author2_role | author author author |
| author_facet | Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cruyt, Frederik\"},{\"Person.name\":\"Sousa, C. A.\"},{\"Person.name\":\"Machado, Manuela D.\"},{\"Person.name\":\"Soares, Eduardo V.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
| datacite.date.Accepted.fl_str_mv | 2017-03-16T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-10-20T09:38:18Z |
| datacite.date.embargoed.fl_str_mv | 2017-10-20T09:38:18Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| datacite.titles.title.fl_str_mv | Improvement of the slide culture technique for the assessment of yeast viability |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
| dc.date.Accepted.fl_str_mv | 2017-03-16T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-10-20T09:38:18Z |
| dc.date.embargoed.fl_str_mv | 2017-10-20T09:38:18Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/46728 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Institute of Brewing & Distilling |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| dc.title.fl_str_mv | Improvement of the slide culture technique for the assessment of yeast viability |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/54cda4ed-a7be-4022-be2d-da3bd0d9ec8f/download |
| id | rum_3df64e7bee1d88bbd63b7f181fefc4b0 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/46728 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/46728 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Institute of Brewing & Distilling |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engInstitute of Brewing & DistillingporThis work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry.application/pdfporImprovement of the slide culture technique for the assessment of yeast viabilityCruyt, FrederikSousa, C. A.Machado, Manuela D.Soares, Eduardo V.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONCruyt, Frederik; Sousa, C. A.; Machado, Manuela D.; Soares, Eduardo V., Improvement of the slide culture technique for the assessment of yeast viability. Journal of the Institute of Brewing, 123(1), 39-44, 2017ISSNIsPartOf0046-9750DOIIsPartOf10.1002/jib.4002017-10-20T09:38:18Z2017-03-162017-08-03T11:19:51Z2017-03-16T00:00:00ZHandlehttps://hdl.handle.net/1822/46728http://purl.org/coar/access_right/c_abf2open accessEthanol stressMicro-colony countSaccharomyces cerevisiaeStarvationViability653012 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/54cda4ed-a7be-4022-be2d-da3bd0d9ec8f/download |
| spellingShingle | Improvement of the slide culture technique for the assessment of yeast viability Cruyt, Frederik Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| status | SINGLETON |
| subject.fl_str_mv | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| title | Improvement of the slide culture technique for the assessment of yeast viability |
| title_full | Improvement of the slide culture technique for the assessment of yeast viability |
| title_fullStr | Improvement of the slide culture technique for the assessment of yeast viability |
| title_full_unstemmed | Improvement of the slide culture technique for the assessment of yeast viability |
| title_short | Improvement of the slide culture technique for the assessment of yeast viability |
| title_sort | Improvement of the slide culture technique for the assessment of yeast viability |
| topic | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| topic_facet | Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability |
| url | https://hdl.handle.net/1822/46728 |
| visible | 1 |