Publicação
The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata
| Resumo: | Candida glabrata biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of C. glabrata mature biofilms, C. glabrata mnn2 was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of -1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of -1,3 glucans in the C. glabrata mnn2. The deletion of the MNN2 gene in C. glabrata induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of -1,3 glucans appears to have a role in this effect. |
|---|---|
| Autores principais: | Rodrigues, Célia Fortuna |
| Outros Autores: | Boas, Diana Patrícia Andrade Vilas; Haynes, Ken; Henriques, Mariana |
| Assunto: | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439477140488192 |
|---|---|
| author | Rodrigues, Célia Fortuna |
| author2 | Boas, Diana Patrícia Andrade Vilas Haynes, Ken Henriques, Mariana |
| author2_role | author author author |
| author_facet | Rodrigues, Célia Fortuna Boas, Diana Patrícia Andrade Vilas Haynes, Ken Henriques, Mariana |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, Célia Fortuna\"},{\"Person.name\":\"Boas, Diana Patrícia Andrade Vilas\"},{\"Person.name\":\"Haynes, Ken\"},{\"Person.name\":\"Henriques, Mariana\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Célia Fortuna Boas, Diana Patrícia Andrade Vilas Haynes, Ken Henriques, Mariana |
| datacite.date.Accepted.fl_str_mv | 2018-10-30T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-11-06T17:04:20Z |
| datacite.date.embargoed.fl_str_mv | 2018-11-06T17:04:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| datacite.titles.title.fl_str_mv | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Rodrigues, Célia Fortuna Boas, Diana Patrícia Andrade Vilas Haynes, Ken Henriques, Mariana |
| dc.date.Accepted.fl_str_mv | 2018-10-30T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-11-06T17:04:20Z |
| dc.date.embargoed.fl_str_mv | 2018-11-06T17:04:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/56779 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| dc.title.fl_str_mv | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Candida glabrata biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of C. glabrata mature biofilms, C. glabrata mnn2 was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of -1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of -1,3 glucans in the C. glabrata mnn2. The deletion of the MNN2 gene in C. glabrata induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of -1,3 glucans appears to have a role in this effect. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/cfd1baa0-9910-4d0d-a149-c6de5b0e62d8/download |
| id | rum_4657b52efbfd2e9ecbcb721b08c8c17d |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/56779 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/56779 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rodrigues, Célia Fortuna Boas, Diana Patrícia Andrade Vilas Haynes, Ken Henriques, Mariana |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porCandida glabrata biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of C. glabrata mature biofilms, C. glabrata mnn2 was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of -1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of -1,3 glucans in the C. glabrata mnn2. The deletion of the MNN2 gene in C. glabrata induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of -1,3 glucans appears to have a role in this effect.application/pdfporThe MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrataRodrigues, Célia FortunaBoas, Diana Patrícia Andrade VilasHaynes, KenHenriques, MarianaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONRodrigues, Célia F.; Vilas Boas, Diana; Haynes, Ken; Henriques, Mariana, The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata. Biomolecules, 8(4), 130, 2018PMID30380780ISSNIsPartOf2218-273XEISSNIsPartOf2218-273XDOIIsPartOf10.3390/biom80401302018-11-06T17:04:20Z2018-10-302018-11-06T16:15:21Z2018-10-30T00:00:00ZHandlehttps://hdl.handle.net/1822/56779http://purl.org/coar/access_right/c_abf2open accessCandida glabrataMNN2 geneantifungalresistancebiofilm matrix1359039 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/cfd1baa0-9910-4d0d-a149-c6de5b0e62d8/download |
| spellingShingle | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata Rodrigues, Célia Fortuna Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| status | SINGLETON |
| subject.fl_str_mv | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| title | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| title_full | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| title_fullStr | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| title_full_unstemmed | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| title_short | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| title_sort | The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata |
| topic | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| topic_facet | Candida glabrata MNN2 gene antifungal resistance biofilm matrix |
| url | https://hdl.handle.net/1822/56779 |
| visible | 1 |