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Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion

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Resumo:The application of conductive materials (CM) has emerged as a strategy to enhance methane production (MP) in anaerobic digestion (AD) systems. This study highlights the potential of sand as an alternative to CM for biomethane production during AD, utilizing complex microbial communities and pure cultures of methanogens. Sand (non-CM) was compared with activated carbon (AC) and nano-zero valent iron (nZVI), both CM, regarding MP kinetic parameters, including lag phase duration, methane production rate (MPR), and maximum MP. Biomethane potential tests revealed that all tested materials improved methane potential, with sand showing significant advancements in the degradation of various substrates, including sewage sludge, glucose, butyrate, and ethanol. Notably, sand led to an increase of up to 16.8 % in the maximum MP from sludge degradation compared to controls without materials, outperforming AC and nZVI. In assays with pure cultures, sand increased the MPR from the hydrogenotrophic Methanobacterium formicicum culture from 9.87 ± 1.97 mM·d1 to 12.45 ± 2.63 mM·d1. No significant effect was observed on MPR from acetoclastic Methanothrix harundinacea. Our findings emphasize the remarkable potential of sand as a low-cost and environmentally friendly alternative to traditional CM to enhance AD efficiency.
Autores principais:Hoffmann, Nicolás
Outros Autores:Braga, Cátia Sofia Neves; Rubilar, Olga; Ciudad, Gustavo; Tortella, Gonzalo; Hermosilla, Edward; Duarte, Maria Salomé; Pereira, Luciana; Salvador, Andreia F.; Martins, Gilberto
Assunto:Anaerobic digestion Conductive materials Sand Methane production Biochemical methane potential tests
Ano:2025
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Hoffmann, Nicolás
author2 Braga, Cátia Sofia Neves
Rubilar, Olga
Ciudad, Gustavo
Tortella, Gonzalo
Hermosilla, Edward
Duarte, Maria Salomé
Pereira, Luciana
Salvador, Andreia F.
Martins, Gilberto
author2_role author
author
author
author
author
author
author
author
author
author_facet Hoffmann, Nicolás
Braga, Cátia Sofia Neves
Rubilar, Olga
Ciudad, Gustavo
Tortella, Gonzalo
Hermosilla, Edward
Duarte, Maria Salomé
Pereira, Luciana
Salvador, Andreia F.
Martins, Gilberto
author_role author
contributor_name_str_mv RepositóriUM - Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Hoffmann, Nicolás\"},{\"Person.name\":\"Braga, Cátia Sofia Neves\"},{\"Person.name\":\"Rubilar, Olga\"},{\"Person.name\":\"Ciudad, Gustavo\"},{\"Person.name\":\"Tortella, Gonzalo\"},{\"Person.name\":\"Hermosilla, Edward\"},{\"Person.name\":\"Duarte, Maria Salomé\"},{\"Person.name\":\"Pereira, Luciana\"},{\"Person.name\":\"Salvador, Andreia F.\"},{\"Person.name\":\"Martins, Gilberto\"}]
datacite.contributors.contributor.contributorName.fl_str_mv RepositóriUM - Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Hoffmann, Nicolás
Braga, Cátia Sofia Neves
Rubilar, Olga
Ciudad, Gustavo
Tortella, Gonzalo
Hermosilla, Edward
Duarte, Maria Salomé
Pereira, Luciana
Salvador, Andreia F.
Martins, Gilberto
datacite.date.Accepted.fl_str_mv 2025-12-01T00:00:00Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
datacite.titles.title.fl_str_mv Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
dc.contributor.none.fl_str_mv RepositóriUM - Universidade do Minho
dc.creator.none.fl_str_mv Hoffmann, Nicolás
Braga, Cátia Sofia Neves
Rubilar, Olga
Ciudad, Gustavo
Tortella, Gonzalo
Hermosilla, Edward
Duarte, Maria Salomé
Pereira, Luciana
Salvador, Andreia F.
Martins, Gilberto
dc.date.Accepted.fl_str_mv 2025-12-01T00:00:00Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/98415
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Elsevier
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.rights.copyright.fl_str_mv openAccess
dc.subject.none.fl_str_mv Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
dc.title.fl_str_mv Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description The application of conductive materials (CM) has emerged as a strategy to enhance methane production (MP) in anaerobic digestion (AD) systems. This study highlights the potential of sand as an alternative to CM for biomethane production during AD, utilizing complex microbial communities and pure cultures of methanogens. Sand (non-CM) was compared with activated carbon (AC) and nano-zero valent iron (nZVI), both CM, regarding MP kinetic parameters, including lag phase duration, methane production rate (MPR), and maximum MP. Biomethane potential tests revealed that all tested materials improved methane potential, with sand showing significant advancements in the degradation of various substrates, including sewage sludge, glucose, butyrate, and ethanol. Notably, sand led to an increase of up to 16.8 % in the maximum MP from sludge degradation compared to controls without materials, outperforming AC and nZVI. In assays with pure cultures, sand increased the MPR from the hydrogenotrophic Methanobacterium formicicum culture from 9.87 ± 1.97 mM·d1 to 12.45 ± 2.63 mM·d1. No significant effect was observed on MPR from acetoclastic Methanothrix harundinacea. Our findings emphasize the remarkable potential of sand as a low-cost and environmentally friendly alternative to traditional CM to enhance AD efficiency.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://repositorium.uminho.pt/bitstreams/a3d134f7-e824-4d77-a180-af187dcb74ad/download
id rum_4e89a698ca320cb87c5f82b6c3be0341
identifier.url.fl_str_mv https://hdl.handle.net/1822/98415
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/98415
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Hoffmann, Nicolás
Braga, Cátia Sofia Neves
Rubilar, Olga
Ciudad, Gustavo
Tortella, Gonzalo
Hermosilla, Edward
Duarte, Maria Salomé
Pereira, Luciana
Salvador, Andreia F.
Martins, Gilberto
publishDate 2025
publisher.none.fl_str_mv Elsevier
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engElsevierengThe application of conductive materials (CM) has emerged as a strategy to enhance methane production (MP) in anaerobic digestion (AD) systems. This study highlights the potential of sand as an alternative to CM for biomethane production during AD, utilizing complex microbial communities and pure cultures of methanogens. Sand (non-CM) was compared with activated carbon (AC) and nano-zero valent iron (nZVI), both CM, regarding MP kinetic parameters, including lag phase duration, methane production rate (MPR), and maximum MP. Biomethane potential tests revealed that all tested materials improved methane potential, with sand showing significant advancements in the degradation of various substrates, including sewage sludge, glucose, butyrate, and ethanol. Notably, sand led to an increase of up to 16.8 % in the maximum MP from sludge degradation compared to controls without materials, outperforming AC and nZVI. In assays with pure cultures, sand increased the MPR from the hydrogenotrophic Methanobacterium formicicum culture from 9.87 ± 1.97 mM·d1 to 12.45 ± 2.63 mM·d1. No significant effect was observed on MPR from acetoclastic Methanothrix harundinacea. Our findings emphasize the remarkable potential of sand as a low-cost and environmentally friendly alternative to traditional CM to enhance AD efficiency.application/pdfengChallenging the conductive paradigm: The unexpected role of sand in anaerobic digestionHoffmann, NicolásBraga, Cátia Sofia NevesRubilar, OlgaCiudad, GustavoTortella, GonzaloHermosilla, EdwardDuarte, Maria SaloméPereira, LucianaSalvador, Andreia F.Martins, GilbertoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONHoffmann, N., Braga, C. S. N., Rubilar, O., Ciudad, G., Tortella, G., Hermosilla, E., Duarte, M. S., Pereira, L., Salvador, A. F., & Martins, G. (2025). Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion. Bioresource Technology, 438, 133163. https://doi.org/10.1016/j.biortech.2025.133163ISSNIsPartOf0960-8524DOIIsPartOf10.1016/j.biortech.2025.133163URLHasVersionhttps://www.sciencedirect.com/science/article/pii/S09608524250113072025-122025-09-01T21:41:49Z2025-12-01T00:00:00ZHandlehttps://hdl.handle.net/1822/98415http://purl.org/coar/access_right/c_abf2open accessAnaerobic digestionConductive materialsSandMethane productionBiochemical methane potential tests2358335 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025-12http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/a3d134f7-e824-4d77-a180-af187dcb74ad/download
spellingShingle Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
Hoffmann, Nicolás
Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
status SINGLETON
subject.fl_str_mv Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
title Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
title_full Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
title_fullStr Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
title_full_unstemmed Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
title_short Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
title_sort Challenging the conductive paradigm: The unexpected role of sand in anaerobic digestion
topic Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
topic_facet Anaerobic digestion
Conductive materials
Sand
Methane production
Biochemical methane potential tests
url https://hdl.handle.net/1822/98415
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