Publicação

Chemical characterization of Sambucus nigra L. flowers aqueous extract and its biological implications

Ver documento

Detalhes bibliográficos
Resumo:The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification of 46 compounds, being quercetin and chlorogenic acid derivatives representative of 86% of the total of phenolic compounds identified in hydrophilic fraction of the aqueous extract. Naringenin (27.2%) was the major compound present in the lipophilic fraction. The antiproliferative effects of the S. nigra extract were evaluated using the colon cancer cell lines RKO, HCT-116, Caco-2 and the extracts antigenotoxic potential was evaluated by the Comet assay in RKO cells. The RKO cells were the most susceptible to S. nigra flower extract (IC50=1250 µg mL1). Moreover, the extract showed antimicrobial activity against Gram-positive bacteria, particularly Staphylococcus aureus and S. epidermidis. These results show that S. nigra-based extracts can be an important dietary source of bioactive phenolic compounds that contribute to health-span improving life quality, demonstrating their potential as nutraceutical, functional foods and/or cosmetic components for therapeutic purposes.
Autores principais:Ferreira-Santos, P.
Outros Autores:Costa, H.; Salvador, Ângelo C.; Silvestre, Armando J. D.; Santos, Sónia A. O.; Rocha, Sílvia M.; Sousa, Ana Margarida; Pereira, Maria Olívia; Pereira-Wilson, Cristina; Rocha, Cristina M.R.; Teixeira, J. A.; Botelho, C. M.
Assunto:Elderflower GC-MS HPLC-MS Chemical profile Phenolic compounds Cytotoxicity Antibacterial activity Antioxidant activity Ciências Naturais::Ciências Biológicas
Ano:2021
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

Registos relacionados

Registos relacionados