Publicação
Marine Collagen/Apatite scaffolds envisaging tissue engineering applications
| Resumo: | Despite the vast investigation and the large amount of products already available in the market to treat the different bone defects there is still a growing need to develop more advanced and complex therapeutic strategies. In this context, a mixture of Marine Hydroxyapatite-Fluorapatite:Collagen (HA-FP:ASC) seems to be a promising solution to overcome these bone defects, specifically, dental defects. HA-FP particles (20–63 μm) were obtained through pyrolysis (950°C, 12 h) of shark teeth (Isurus oxyrinchus, P. glauca), and Type I collagen was isolated from Prionace glauca skin as previously described (1). After the steps of purification, collagen was solubilized in 0.5 M acetic acid and HA-FP added producing three different formulations: were produced, 30:70, 50:50 and 70:30 of HA-FP:ASC, respectively. EDC/NHS and HMDI binding agents were used to stabilize the produced scaffolds. Mechanical properties were evaluated by compression tests. SEM analysis allowed observing the mineral deposition, after immersion in simulated body fluid and also permitted to evaluate how homogenous was the distribution of HA-FP in the different scaffold formulations, also confirmed by μ-CT assay. It was readily visible by Cytotoxicity and life/dead CLSM assays that cells were able to adhere and proliferate in the produced scaffolds. Scaffolds crosslinked with EDC/NHS showed lower cytotoxicity, being the ones chosen for further cellular evaluation. |
|---|---|
| Autores principais: | Diogo, G. S. |
| Outros Autores: | Lopez, E.; González, P.; Serra, J.; Perez-Martin, R. I.; Sotelo, C. G.; Canadas, Raphael Faustino; Silva, Tiago H.; Silva, Joana M.; Reis, R. L. |
| Assunto: | Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439708313747456 |
|---|---|
| author | Diogo, G. S. |
| author2 | Lopez, E. González, P. Serra, J. Perez-Martin, R. I. Sotelo, C. G. Canadas, Raphael Faustino Silva, Tiago H. Silva, Joana M. Reis, R. L. |
| author2_role | author author author author author author author author author |
| author_facet | Diogo, G. S. Lopez, E. González, P. Serra, J. Perez-Martin, R. I. Sotelo, C. G. Canadas, Raphael Faustino Silva, Tiago H. Silva, Joana M. Reis, R. L. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Diogo, G. S.\"},{\"Person.name\":\"Lopez, E.\"},{\"Person.name\":\"González, P.\"},{\"Person.name\":\"Serra, J.\"},{\"Person.name\":\"Perez-Martin, R. I.\"},{\"Person.name\":\"Sotelo, C. G.\"},{\"Person.name\":\"Canadas, Raphael Faustino\"},{\"Person.name\":\"Silva, Tiago H.\"},{\"Person.name\":\"Silva, Joana M.\"},{\"Person.name\":\"Reis, R. L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Diogo, G. S. Lopez, E. González, P. Serra, J. Perez-Martin, R. I. Sotelo, C. G. Canadas, Raphael Faustino Silva, Tiago H. Silva, Joana M. Reis, R. L. |
| datacite.date.Accepted.fl_str_mv | 2015-08-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| datacite.titles.title.fl_str_mv | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Diogo, G. S. Lopez, E. González, P. Serra, J. Perez-Martin, R. I. Sotelo, C. G. Canadas, Raphael Faustino Silva, Tiago H. Silva, Joana M. Reis, R. L. |
| dc.date.Accepted.fl_str_mv | 2015-08-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/41007 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Mary Ann Liebert |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| dc.title.fl_str_mv | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | Despite the vast investigation and the large amount of products already available in the market to treat the different bone defects there is still a growing need to develop more advanced and complex therapeutic strategies. In this context, a mixture of Marine Hydroxyapatite-Fluorapatite:Collagen (HA-FP:ASC) seems to be a promising solution to overcome these bone defects, specifically, dental defects. HA-FP particles (20–63 μm) were obtained through pyrolysis (950°C, 12 h) of shark teeth (Isurus oxyrinchus, P. glauca), and Type I collagen was isolated from Prionace glauca skin as previously described (1). After the steps of purification, collagen was solubilized in 0.5 M acetic acid and HA-FP added producing three different formulations: were produced, 30:70, 50:50 and 70:30 of HA-FP:ASC, respectively. EDC/NHS and HMDI binding agents were used to stabilize the produced scaffolds. Mechanical properties were evaluated by compression tests. SEM analysis allowed observing the mineral deposition, after immersion in simulated body fluid and also permitted to evaluate how homogenous was the distribution of HA-FP in the different scaffold formulations, also confirmed by μ-CT assay. It was readily visible by Cytotoxicity and life/dead CLSM assays that cells were able to adhere and proliferate in the produced scaffolds. Scaffolds crosslinked with EDC/NHS showed lower cytotoxicity, being the ones chosen for further cellular evaluation. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | other |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/46c7a3d9-4497-42ac-8a17-9c7d247d81d1/download |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/41007 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/41007 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Diogo, G. S. Lopez, E. González, P. Serra, J. Perez-Martin, R. I. Sotelo, C. G. Canadas, Raphael Faustino Silva, Tiago H. Silva, Joana M. Reis, R. L. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Mary Ann Liebert |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
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| spelling | engMary Ann LiebertporDespite the vast investigation and the large amount of products already available in the market to treat the different bone defects there is still a growing need to develop more advanced and complex therapeutic strategies. In this context, a mixture of Marine Hydroxyapatite-Fluorapatite:Collagen (HA-FP:ASC) seems to be a promising solution to overcome these bone defects, specifically, dental defects. HA-FP particles (20–63 μm) were obtained through pyrolysis (950°C, 12 h) of shark teeth (Isurus oxyrinchus, P. glauca), and Type I collagen was isolated from Prionace glauca skin as previously described (1). After the steps of purification, collagen was solubilized in 0.5 M acetic acid and HA-FP added producing three different formulations: were produced, 30:70, 50:50 and 70:30 of HA-FP:ASC, respectively. EDC/NHS and HMDI binding agents were used to stabilize the produced scaffolds. Mechanical properties were evaluated by compression tests. SEM analysis allowed observing the mineral deposition, after immersion in simulated body fluid and also permitted to evaluate how homogenous was the distribution of HA-FP in the different scaffold formulations, also confirmed by μ-CT assay. It was readily visible by Cytotoxicity and life/dead CLSM assays that cells were able to adhere and proliferate in the produced scaffolds. Scaffolds crosslinked with EDC/NHS showed lower cytotoxicity, being the ones chosen for further cellular evaluation.application/pdfporMarine Collagen/Apatite scaffolds envisaging tissue engineering applicationsDiogo, G. S.Lopez, E.González, P.Serra, J.Perez-Martin, R. I.Sotelo, C. G.Canadas, Raphael FaustinoSilva, Tiago H.Silva, Joana M.Reis, R. L.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONDiogo G. S., Lopez E., González P., Serra J., Perez-Martin R. I., Sotelo C. G., Canadas R. F., Silva T. H., Moreira-Silva J., Reis R. L. Marine Collagen/Apatite Scaffolds Envisaging Tissue Engineering Applications., TISSUE ENGINEERING PART A, Vol. 21, Issue S1, pp. S210-S210, doi:10.1089/ten.tea.2015.5000.abstracts, 2015ISSNIsPartOf2152-49472015-082016-03-04T14:28:48Z2015-08-01T00:00:00ZHandlehttps://hdl.handle.net/1822/41007http://purl.org/coar/access_right/c_16ecrestricted accessCollagenHydroxiapatiteMarineScaffoldshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Engenharia e Tecnologia::Biotecnologia Industrial195568 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorium.uminho.pt/bitstreams/46c7a3d9-4497-42ac-8a17-9c7d247d81d1/download |
| spellingShingle | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications Diogo, G. S. Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| status | SINGLETON |
| subject.fl_str_mv | Collagen Hydroxiapatite Marine Scaffolds |
| subject.other.fl_str_mv | Engenharia e Tecnologia::Biotecnologia Industrial |
| title | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| title_full | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| title_fullStr | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| title_full_unstemmed | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| title_short | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| title_sort | Marine Collagen/Apatite scaffolds envisaging tissue engineering applications |
| topic | Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| topic_facet | Collagen Hydroxiapatite Marine Scaffolds Engenharia e Tecnologia::Biotecnologia Industrial |
| url | https://hdl.handle.net/1822/41007 |
| visible | 1 |