Publicação

Recruitment of antigen-specific CD8+ T cells in response to infection is markedly efficient

Detalhes bibliográficos
Resumo:	The magnitude of antigen-specific CD8+ T cell responses is not fixed but correlates with the severity of infection. Although by definition T cell response size is the product of both the capacity to recruit naïve T cells (clonal selection) and their subsequent proliferation (clonal expansion), it remains undefined how these two factors regulate antigen-specific T cell responses. We determined the relative contribution of recruitment and expansion by labeling naïve T cells with unique genetic tags and transferring them into mice. Under disparate infection conditions with different pathogens and doses, recruitment of antigen-specific T cells was near constant and close to complete. Thus, naïve T cell recruitment is highly efficient, and the magnitude of antigen-specific CD8+ T cell responses is primarily controlled by clonal expansion.
Autores principais:	Heijst, Jeroen W. J. van
Outros Autores:	Gerlach, Carmen; Swart, Erwin; Sie, Daoud; Alves, Cláudio Nunes; Kerkhoven, Ron M.; Arens, Ramon; Correia-Neves, M; Schepers, Koen; Schumacher, Ton N. M.
Assunto:	Adoptive Transfer Ampicillin Animals Anti-Bacterial Agents Antigens Antigens, Bacterial Antigens, Viral CD8-Positive T-Lymphocytes Dendritic Cells Epitopes Genes, T-Cell Receptor alpha Genes, T-Cell Receptor beta Influenza A virus Listeriosis Lymphocyte Count Mice Mice, Inbred C57BL Mice, Transgenic Molecular Sequence Data Orthomyxoviridae Infections Ovalbumin Receptors, Antigen, T-Cell, alpha-beta Spleen Vaccinia Virus Diseases Lymphocyte Activation
Ano:	2009
País:	Portugal
Tipo de documento:	artigo
Tipo de acesso:	acesso restrito
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	The magnitude of antigen-specific CD8+ T cell responses is not fixed but correlates with the severity of infection. Although by definition T cell response size is the product of both the capacity to recruit naïve T cells (clonal selection) and their subsequent proliferation (clonal expansion), it remains undefined how these two factors regulate antigen-specific T cell responses. We determined the relative contribution of recruitment and expansion by labeling naïve T cells with unique genetic tags and transferring them into mice. Under disparate infection conditions with different pathogens and doses, recruitment of antigen-specific T cells was near constant and close to complete. Thus, naïve T cell recruitment is highly efficient, and the magnitude of antigen-specific CD8+ T cell responses is primarily controlled by clonal expansion.