Publicação
Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
| Resumo: | Bacterial vaginosis (BV) is associated with serious gynaecologic and obstetric complications. The hallmark of BV is the presence of a polymicrobial biofilm on the vaginal epithelium, but BV aetiology is still a matter of debate. We have previously developed an in vitro biofilm model that included three BV-associated species, but, up to now, no studies are available whereby more bacterial species are grown together to better mimic the in vivo situation. Herein, we characterized the first polymicrobial BV biofilm consisting of six cultivable BV-associated species by using both in vitro and ex vivo vaginal tissue models. Both models revealed that the six species were able to incorporate the polymicrobial biofilm, at different bacterial concentrations. As it has been thought that this polymicrobial biofilm may increase the survival of BV-associated species when exposed to antibiotics, we also assessed if the Thymbra capitata essential oil (EO), which has recently been shown to be highly bactericidal against several Gardnerella species, could maintain its anti-biofilm activity against this polymicrobial biofilm. Under our experimental conditions, T. capitata EO exhibited a high antibacterial effect against polymicrobial biofilms, in both tested models, with a significant reduction in the biofilm biomass and the number of culturable cells. Overall, this study shows that six BV-associated species can grow together and form a biofilm both in vitro and when using an ex vivo model. Moreover, the data obtained herein should be considered in further applications of T. capitata EO as an antimicrobial agent fighting BV. |
|---|---|
| Autores principais: | Rosca, Aliona |
| Outros Autores: | Castro, Joana Isabel Reis; Sousa, Lúcia; França, Ângela Maria Oliveira Sousa; Cavaleiro, Carlos; Salgueiro, Lígia; Cerca, Nuno |
| Assunto: | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439791735308288 |
|---|---|
| author | Rosca, Aliona |
| author2 | Castro, Joana Isabel Reis Sousa, Lúcia França, Ângela Maria Oliveira Sousa Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno |
| author2_role | author author author author author author |
| author_facet | Rosca, Aliona Castro, Joana Isabel Reis Sousa, Lúcia França, Ângela Maria Oliveira Sousa Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rosca, Aliona\"},{\"Person.name\":\"Castro, Joana Isabel Reis\"},{\"Person.name\":\"Sousa, Lúcia\"},{\"Person.name\":\"França, Ângela Maria Oliveira Sousa\"},{\"Person.name\":\"Cavaleiro, Carlos\"},{\"Person.name\":\"Salgueiro, Lígia\"},{\"Person.name\":\"Cerca, Nuno\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rosca, Aliona Castro, Joana Isabel Reis Sousa, Lúcia França, Ângela Maria Oliveira Sousa Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno |
| datacite.date.Accepted.fl_str_mv | 2022-05-04T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-02-13T12:40:12Z |
| datacite.date.embargoed.fl_str_mv | 2023-02-13T12:40:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| datacite.titles.title.fl_str_mv | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Rosca, Aliona Castro, Joana Isabel Reis Sousa, Lúcia França, Ângela Maria Oliveira Sousa Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno |
| dc.date.Accepted.fl_str_mv | 2022-05-04T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-02-13T12:40:12Z |
| dc.date.embargoed.fl_str_mv | 2023-02-13T12:40:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/82633 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers Media S.A. |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| dc.title.fl_str_mv | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bacterial vaginosis (BV) is associated with serious gynaecologic and obstetric complications. The hallmark of BV is the presence of a polymicrobial biofilm on the vaginal epithelium, but BV aetiology is still a matter of debate. We have previously developed an in vitro biofilm model that included three BV-associated species, but, up to now, no studies are available whereby more bacterial species are grown together to better mimic the in vivo situation. Herein, we characterized the first polymicrobial BV biofilm consisting of six cultivable BV-associated species by using both in vitro and ex vivo vaginal tissue models. Both models revealed that the six species were able to incorporate the polymicrobial biofilm, at different bacterial concentrations. As it has been thought that this polymicrobial biofilm may increase the survival of BV-associated species when exposed to antibiotics, we also assessed if the Thymbra capitata essential oil (EO), which has recently been shown to be highly bactericidal against several Gardnerella species, could maintain its anti-biofilm activity against this polymicrobial biofilm. Under our experimental conditions, T. capitata EO exhibited a high antibacterial effect against polymicrobial biofilms, in both tested models, with a significant reduction in the biofilm biomass and the number of culturable cells. Overall, this study shows that six BV-associated species can grow together and form a biofilm both in vitro and when using an ex vivo model. Moreover, the data obtained herein should be considered in further applications of T. capitata EO as an antimicrobial agent fighting BV. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/6645d10f-e777-4942-a13b-e9e488529c81/download |
| id | rum_7fbf8399ba9849eec81ecbebd13b76db |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/82633 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/82633 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rosca, Aliona Castro, Joana Isabel Reis Sousa, Lúcia França, Ângela Maria Oliveira Sousa Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Frontiers Media S.A. |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engFrontiers Media S.A.porBacterial vaginosis (BV) is associated with serious gynaecologic and obstetric complications. The hallmark of BV is the presence of a polymicrobial biofilm on the vaginal epithelium, but BV aetiology is still a matter of debate. We have previously developed an in vitro biofilm model that included three BV-associated species, but, up to now, no studies are available whereby more bacterial species are grown together to better mimic the in vivo situation. Herein, we characterized the first polymicrobial BV biofilm consisting of six cultivable BV-associated species by using both in vitro and ex vivo vaginal tissue models. Both models revealed that the six species were able to incorporate the polymicrobial biofilm, at different bacterial concentrations. As it has been thought that this polymicrobial biofilm may increase the survival of BV-associated species when exposed to antibiotics, we also assessed if the Thymbra capitata essential oil (EO), which has recently been shown to be highly bactericidal against several Gardnerella species, could maintain its anti-biofilm activity against this polymicrobial biofilm. Under our experimental conditions, T. capitata EO exhibited a high antibacterial effect against polymicrobial biofilms, in both tested models, with a significant reduction in the biofilm biomass and the number of culturable cells. Overall, this study shows that six BV-associated species can grow together and form a biofilm both in vitro and when using an ex vivo model. Moreover, the data obtained herein should be considered in further applications of T. capitata EO as an antimicrobial agent fighting BV.application/pdfporSix bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oilRosca, AlionaCastro, Joana Isabel ReisSousa, LúciaFrança, Ângela Maria Oliveira SousaCavaleiro, CarlosSalgueiro, LígiaCerca, NunoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONRosca, A. S., Castro, J., Sousa, L. G. V., França, A., Cavaleiro, C., Salgueiro, L., & Cerca, N. (2022, May 4). Six Bacterial Vaginosis-Associated Species Can Form an In Vitro and Ex Vivo Polymicrobial Biofilm That Is Susceptible to Thymbra capitata Essential Oil. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2022.824860PMID35601098ARTICLENUMBER824860ISSNIsPartOf2235-2988DOIIsPartOf10.3389/fcimb.2022.8248602023-02-13T12:40:12Z2022-05-042023-02-13T11:24:15Z2022-05-04T00:00:00ZHandlehttps://hdl.handle.net/1822/82633http://purl.org/coar/access_right/c_abf2open accessbacterial vaginosis (BV)polymicrobial biofilmsalternative therapyessential oilsmicrobial interactions1113745 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/6645d10f-e777-4942-a13b-e9e488529c81/download |
| spellingShingle | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil Rosca, Aliona bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| status | SINGLETON |
| subject.fl_str_mv | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| title | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| title_full | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| title_fullStr | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| title_full_unstemmed | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| title_short | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| title_sort | Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil |
| topic | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| topic_facet | bacterial vaginosis (BV) polymicrobial biofilms alternative therapy essential oils microbial interactions |
| url | https://hdl.handle.net/1822/82633 |
| visible | 1 |