Publicação
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
| Resumo: | Flavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcriptmetabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcriptmetabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols. |
|---|---|
| Autores principais: | Teixeira, António |
| Outros Autores: | Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, H. |
| Assunto: | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439883321081856 |
|---|---|
| author | Teixeira, António |
| author2 | Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
| author2_role | author author author author author author |
| author_facet | Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Teixeira, António\"},{\"Person.name\":\"Martins, Viviana\"},{\"Person.name\":\"Frusciante, Sarah\"},{\"Person.name\":\"Cruz, Telmo\"},{\"Person.name\":\"Noronha, Henrique\"},{\"Person.name\":\"Diretto, Gianfranco\"},{\"Person.name\":\"Gerós, H.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
| datacite.date.Accepted.fl_str_mv | 2020-06-17T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-06-21T21:23:27Z |
| datacite.date.embargoed.fl_str_mv | 2020-06-21T21:23:27Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| datacite.titles.title.fl_str_mv | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
| dc.date.Accepted.fl_str_mv | 2020-06-17T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-06-21T21:23:27Z |
| dc.date.embargoed.fl_str_mv | 2020-06-21T21:23:27Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/65697 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers Media |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| dc.title.fl_str_mv | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Flavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcriptmetabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcriptmetabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/82b57fbb-9c32-46e2-a9d0-546d14315d7f/download |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/65697 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/65697 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Frontiers Media |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engFrontiers MediaporFlavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcriptmetabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcriptmetabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols.application/pdfporFlavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathwaysTeixeira, AntónioMartins, VivianaFrusciante, SarahCruz, TelmoNoronha, HenriqueDiretto, GianfrancoGerós, H.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONTeixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni, Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways. Frontiers in Plant Science, 11(896), 2020ISSNIsPartOf1664-462XDOIIsPartOf10.3389/fpls.2020.008962020-06-21T21:23:27Z2020-06-172020-06-20T09:59:31Z2020-06-17T00:00:00ZHandlehttps://hdl.handle.net/1822/65697http://purl.org/coar/access_right/c_abf2open accessVitis viniferaflavescence doreebiotic stressleaf yellowingisoprenoids biosynthesisplant pathogens2743632 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/82b57fbb-9c32-46e2-a9d0-546d14315d7f/download |
| spellingShingle | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways Teixeira, António Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| status | SINGLETON |
| subject.fl_str_mv | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| title | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| title_full | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| title_fullStr | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| title_full_unstemmed | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| title_short | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| title_sort | Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
| topic | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| topic_facet | Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens |
| url | https://hdl.handle.net/1822/65697 |
| visible | 1 |