Publicação
Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14
| Resumo: | Lactobacillus acidophilus is a probiotic lactic acid bacterium used in food and dietary supplements for many years. However, despite its importance for industrial development and recognized health-promoting effects, no genome-scale metabolic model has been reported. A GSM model for L. acidophilus La-14 was developed, accounting 494 genes and 783 reactions. A genome annotation was performed to identify the metabolic potential of the bacterium. The biomass composition was determined based on information available in literature and previously published models. The model was validated by comparing in silico simulations with experimental data, regarding the aerobic and anaerobic growth. The reconstruction of the metabolic model has confirmed the fastidious requirements of L. acidophilus for amino acids, fatty acids, and vitamins. This model can be used for a better understanding of the metabolism of this bacterium and identification of industrially desirable compounds. |
|---|---|
| Autores principais: | Cunha, Emanuel |
| Outros Autores: | Zeidan, Ahmad; Dias, Oscar |
| Assunto: | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438970480099328 |
|---|---|
| author | Cunha, Emanuel |
| author2 | Zeidan, Ahmad Dias, Oscar |
| author2_role | author author |
| author_facet | Cunha, Emanuel Zeidan, Ahmad Dias, Oscar |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cunha, Emanuel\"},{\"Person.name\":\"Zeidan, Ahmad\"},{\"Person.name\":\"Dias, Oscar\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Cunha, Emanuel Zeidan, Ahmad Dias, Oscar |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-10-25T10:16:43Z |
| datacite.date.embargoed.fl_str_mv | 2021-10-25T10:16:43Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| datacite.titles.title.fl_str_mv | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Cunha, Emanuel Zeidan, Ahmad Dias, Oscar |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-10-25T10:16:43Z |
| dc.date.embargoed.fl_str_mv | 2021-10-25T10:16:43Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/74498 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer International Publishing |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| dc.title.fl_str_mv | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | Lactobacillus acidophilus is a probiotic lactic acid bacterium used in food and dietary supplements for many years. However, despite its importance for industrial development and recognized health-promoting effects, no genome-scale metabolic model has been reported. A GSM model for L. acidophilus La-14 was developed, accounting 494 genes and 783 reactions. A genome annotation was performed to identify the metabolic potential of the bacterium. The biomass composition was determined based on information available in literature and previously published models. The model was validated by comparing in silico simulations with experimental data, regarding the aerobic and anaerobic growth. The reconstruction of the metabolic model has confirmed the fastidious requirements of L. acidophilus for amino acids, fatty acids, and vitamins. This model can be used for a better understanding of the metabolism of this bacterium and identification of industrially desirable compounds. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/eac9d17b-3e29-41fe-ac06-e5cdb3f4b57f/download |
| id | rum_86cff960eb8104783d2f0fe8b6060b6a |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/74498 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/74498 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Cunha, Emanuel Zeidan, Ahmad Dias, Oscar |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Springer International Publishing |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringer International PublishingporLactobacillus acidophilus is a probiotic lactic acid bacterium used in food and dietary supplements for many years. However, despite its importance for industrial development and recognized health-promoting effects, no genome-scale metabolic model has been reported. A GSM model for L. acidophilus La-14 was developed, accounting 494 genes and 783 reactions. A genome annotation was performed to identify the metabolic potential of the bacterium. The biomass composition was determined based on information available in literature and previously published models. The model was validated by comparing in silico simulations with experimental data, regarding the aerobic and anaerobic growth. The reconstruction of the metabolic model has confirmed the fastidious requirements of L. acidophilus for amino acids, fatty acids, and vitamins. This model can be used for a better understanding of the metabolism of this bacterium and identification of industrially desirable compounds.application/pdfporTowards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14Cunha, EmanuelZeidan, AhmadDias, OscarHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONCunha, Emanuel; Zeidan, Ahmad; Dias, Oscar, Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14. PACBB 2020 - 14th International Conference Practical Applications of Computational Biology & Bioinformatics. Vol. Advances in Intelligent Systems and Computing 1240, L'Aquila, Italy, Oct 7-9, Springer International Publishing, 205-214, 2021. ISBN: 978-3-030-54568-0ISBNIsPartOf978-3-030-54568-0ISSNIsPartOf2194-5357DOIIsPartOf10.1007/978-3-030-54568-0_212021-10-25T10:16:43Z20212021-10-25T08:44:59Z2021-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/74498http://purl.org/coar/access_right/c_abf2open accessGenome-scale metabolic modelLactic acid bacteriaProbioticLactobacillus acidophilus La-14Metabolic reconstructionhttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Biológicas521865 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/eac9d17b-3e29-41fe-ac06-e5cdb3f4b57f/download |
| spellingShingle | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 Cunha, Emanuel Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| status | SINGLETON |
| subject.fl_str_mv | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Biológicas |
| title | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| title_full | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| title_fullStr | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| title_full_unstemmed | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| title_short | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| title_sort | Towards the reconstruction of the genome-scale metabolic model of Lactobacillus acidophilus La-14 |
| topic | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| topic_facet | Genome-scale metabolic model Lactic acid bacteria Probiotic Lactobacillus acidophilus La-14 Metabolic reconstruction Ciências Naturais::Ciências Biológicas |
| url | https://hdl.handle.net/1822/74498 |
| visible | 1 |