Publicação
Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control
| Resumo: | Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery. |
|---|---|
| Autores principais: | Vinayakumar, K. B. |
| Outros Autores: | Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna M. |
| Assunto: | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438274680717312 |
|---|---|
| author | Vinayakumar, K. B. |
| author2 | Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
| author2_role | author author author author author |
| author_facet | Vinayakumar, K. B. Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Vinayakumar, K. B.\"},{\"Person.name\":\"Silva, Maria Daniela\"},{\"Person.name\":\"Martins, Artur\"},{\"Person.name\":\"Mundy, Stephen\"},{\"Person.name\":\"González-Losada, Pedro\"},{\"Person.name\":\"Sillankorva, Sanna M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Vinayakumar, K. B. Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
| datacite.date.Accepted.fl_str_mv | 2023-06-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-06-20T08:40:40Z |
| datacite.date.embargoed.fl_str_mv | 2023-06-20T08:40:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| datacite.titles.title.fl_str_mv | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Vinayakumar, K. B. Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
| dc.date.Accepted.fl_str_mv | 2023-06-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-06-20T08:40:40Z |
| dc.date.embargoed.fl_str_mv | 2023-06-20T08:40:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/85094 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| dc.title.fl_str_mv | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/3ff9b921-0049-4476-8a21-9fb7116a4647/download |
| id | rum_8ec1e2d27fdc2f747e744c094e0825a3 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/85094 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/85094 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Vinayakumar, K. B. Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engWileyporAdditive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery.application/pdfporLevofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm controlVinayakumar, K. B.Silva, Maria DanielaMartins, ArturMundy, StephenGonzález-Losada, PedroSillankorva, Sanna M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONVinayakumar, K. B.; Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna M., Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control. Advanced Therapeutics, 6(6), 2200320, 2023ISSNIsPartOf2366-3987DOIIsPartOf10.1002/adtp.2022003202023-06-20T08:40:40Z2023-062023-06-17T09:08:07Z2023-06-01T00:00:00ZHandlehttps://hdl.handle.net/1822/85094http://purl.org/coar/access_right/c_abf2open access3D printingbiofilmsKlebsiella pneumoniaelevofloxacinmicroneedlespolyvinyl alcohol3014501 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/3ff9b921-0049-4476-8a21-9fb7116a4647/download |
| spellingShingle | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control Vinayakumar, K. B. 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| status | SINGLETON |
| subject.fl_str_mv | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| title | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| title_full | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| title_fullStr | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| title_full_unstemmed | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| title_short | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| title_sort | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
| topic | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| topic_facet | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
| url | https://hdl.handle.net/1822/85094 |
| visible | 1 |