Publicação
Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications
| Resumo: | AIM: Utilize the dual composition strategy to increase the pore size and solve the low cell infiltration capacity on random nanofiber meshes, an intrinsic limitation of electrospun scaffolds for tissue engineering applications. MATERIALS & METHODS: Polycaprolactone and poly(ethylene oxide) solutions were electrospun simultaneously to obtain a dual composition nanofiber mesh. Selective dissolution of the poly(ethylene oxide) nanofiber fraction was performed. The biologic performance of these enhanced pore size nanofibrous structures was assessed with human osteoblastic cells. RESULTS: The electrospun nanofiber meshes, after the poly(ethylene oxide) dissolution, showed statistically significant larger pore sizes when compared with polycaprolactone nanofiber meshes with a similar polycaprolactone volume fraction. This was also confirmed by interferometric optical profilometry. Using scanning electron microscopy and laser scanning confocal microscopy, it was observed that osteoblastic cells could penetrate into the nanofibrous structure and migrate into the opposite and unseeded side of the mesh. CONCLUSION: An electrospun mesh was created with sufficient pore size to allow cell infiltration into its structure, thus resulting in a fully populated construct appropriate for 3D tissue engineering applications. |
|---|---|
| Autores principais: | Guimarães, Ana Cristina Silva Esperança |
| Outros Autores: | Martins, Albino; Pinho, Elisabete D.; Faria, Susana; Reis, R. L.; Neves, N. M. |
| Assunto: | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438020315054080 |
|---|---|
| author | Guimarães, Ana Cristina Silva Esperança |
| author2 | Martins, Albino Pinho, Elisabete D. Faria, Susana Reis, R. L. Neves, N. M. |
| author2_role | author author author author author |
| author_facet | Guimarães, Ana Cristina Silva Esperança Martins, Albino Pinho, Elisabete D. Faria, Susana Reis, R. L. Neves, N. M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guimarães, Ana Cristina Silva Esperança\"},{\"Person.name\":\"Martins, Albino\"},{\"Person.name\":\"Pinho, Elisabete D.\"},{\"Person.name\":\"Faria, Susana\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Neves, N. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Guimarães, Ana Cristina Silva Esperança Martins, Albino Pinho, Elisabete D. Faria, Susana Reis, R. L. Neves, N. M. |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-10-16T10:25:47Z |
| datacite.date.embargoed.fl_str_mv | 2012-10-16T10:25:47Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| datacite.titles.title.fl_str_mv | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Guimarães, Ana Cristina Silva Esperança Martins, Albino Pinho, Elisabete D. Faria, Susana Reis, R. L. Neves, N. M. |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-10-16T10:25:47Z |
| dc.date.embargoed.fl_str_mv | 2012-10-16T10:25:47Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/20427 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Future Medicine Ltd |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| dc.title.fl_str_mv | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | AIM: Utilize the dual composition strategy to increase the pore size and solve the low cell infiltration capacity on random nanofiber meshes, an intrinsic limitation of electrospun scaffolds for tissue engineering applications. MATERIALS & METHODS: Polycaprolactone and poly(ethylene oxide) solutions were electrospun simultaneously to obtain a dual composition nanofiber mesh. Selective dissolution of the poly(ethylene oxide) nanofiber fraction was performed. The biologic performance of these enhanced pore size nanofibrous structures was assessed with human osteoblastic cells. RESULTS: The electrospun nanofiber meshes, after the poly(ethylene oxide) dissolution, showed statistically significant larger pore sizes when compared with polycaprolactone nanofiber meshes with a similar polycaprolactone volume fraction. This was also confirmed by interferometric optical profilometry. Using scanning electron microscopy and laser scanning confocal microscopy, it was observed that osteoblastic cells could penetrate into the nanofibrous structure and migrate into the opposite and unseeded side of the mesh. CONCLUSION: An electrospun mesh was created with sufficient pore size to allow cell infiltration into its structure, thus resulting in a fully populated construct appropriate for 3D tissue engineering applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/18ece334-13ad-455d-98eb-cb648f237eac/download |
| id | rum_9cbb861ae6eea4d9a3c16eb4e7a0d9aa |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/20427 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/20427 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Guimarães, Ana Cristina Silva Esperança Martins, Albino Pinho, Elisabete D. Faria, Susana Reis, R. L. Neves, N. M. |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Future Medicine Ltd |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engFuture Medicine LtdengAIM: Utilize the dual composition strategy to increase the pore size and solve the low cell infiltration capacity on random nanofiber meshes, an intrinsic limitation of electrospun scaffolds for tissue engineering applications. MATERIALS & METHODS: Polycaprolactone and poly(ethylene oxide) solutions were electrospun simultaneously to obtain a dual composition nanofiber mesh. Selective dissolution of the poly(ethylene oxide) nanofiber fraction was performed. The biologic performance of these enhanced pore size nanofibrous structures was assessed with human osteoblastic cells. RESULTS: The electrospun nanofiber meshes, after the poly(ethylene oxide) dissolution, showed statistically significant larger pore sizes when compared with polycaprolactone nanofiber meshes with a similar polycaprolactone volume fraction. This was also confirmed by interferometric optical profilometry. Using scanning electron microscopy and laser scanning confocal microscopy, it was observed that osteoblastic cells could penetrate into the nanofibrous structure and migrate into the opposite and unseeded side of the mesh. CONCLUSION: An electrospun mesh was created with sufficient pore size to allow cell infiltration into its structure, thus resulting in a fully populated construct appropriate for 3D tissue engineering applications.application/pdfporSolving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applicationsGuimarães, Ana Cristina Silva EsperançaMartins, AlbinoPinho, Elisabete D.Faria, SusanaReis, R. L.Neves, N. M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONGuimarães, A., Martins, A., Pinho, E. D., Faria, S., Reis, R. L., & Neves, N. M. (2010). Solving Cell Infiltration Limitations of Electrospun Nanofiber Meshes for Tissue Engineering Applications. Nanomedicine, 5(4), 539–554. https://doi.org/10.2217/nnm.10.31PMID20528450ISSNIsPartOf1743-5889DOIIsPartOf10.2217/NNM.10.312012-10-16T10:25:47Z20102010-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/20427http://purl.org/coar/access_right/c_abf2open accessbiodegradable scaffoldcell penetrationelectrospun nanofiber meshpore sizetissue enginnering1669212 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/18ece334-13ad-455d-98eb-cb648f237eac/download |
| spellingShingle | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications Guimarães, Ana Cristina Silva Esperança biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| status | SINGLETON |
| subject.fl_str_mv | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| title | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| title_full | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| title_fullStr | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| title_full_unstemmed | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| title_short | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| title_sort | Solving cell infiltration limitations of electrospun nanofiber meshes for tissue engineering applications |
| topic | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| topic_facet | biodegradable scaffold cell penetration electrospun nanofiber mesh pore size tissue enginnering |
| url | https://hdl.handle.net/1822/20427 |
| visible | 1 |