Publicação
Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA)
| Resumo: | Polymicrobial biofilms are an understudied and a clinically relevant problem. This study evaluates the interaction between C. albicans, and methicillin- susceptible (MSSA) and resistant (MRSA) S. aureus growing in single- and dual-species biofilms. Single and dual species adhesion (90 min) and biofilms (12, 24, and 48 h) were evaluated by complementary methods: counting colony-forming units (CFU mL-1), XTT-reduction, and crystal violet staining (CV). The secretion of hydrolytic enzymes by the 48 h biofilms was also evaluated using fluorimetric kits. Scanning electron microscopy (SEM) was used to assess biofilm structure. The results from quantification assays were compared using two-way ANOVAs with Tukey post-hoc tests, while data from enzymatic activities were analyzed by one-way Welch-ANOVA followed by Games-Howell post hoc test ( = 0.05). C. albicans, MSSA and MRSA were able to adhere and to form biofilm in both single or mixed cultures. In general, all microorganisms in both growth conditions showed a gradual increase in the number of cells and metabolic activity over time, reaching peak values between 12 h and 48 h (<0.05). C. albicans single- and dual-biofilms had significantly higher total biomass values (<0.05) than single biofilms of bacteria. Except for single MRSA biofilms, all microorganisms in both growth conditions secreted proteinase and phospholipase-C. SEM images revealed extensive adherence of bacteria to hyphal elements of C. albicans. C. albicans, MSSA, and MRSA can co-exist in biofilms without antagonism and in an apparent synergistic effect, with bacteria cells preferentially associated to C. albicans hyphal forms. |
|---|---|
| Autores principais: | Zago, Chaiene Evelin |
| Outros Autores: | Silva, Sónia Carina; Sanitá, Paula Volpato; Barbugli, Paula Aboud; Dias, Carla Maria Improta; Lordello, Virgínia Barreto; Vergani, Carlos Eduardo |
| Assunto: | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439352313806848 |
|---|---|
| author | Zago, Chaiene Evelin |
| author2 | Silva, Sónia Carina Sanitá, Paula Volpato Barbugli, Paula Aboud Dias, Carla Maria Improta Lordello, Virgínia Barreto Vergani, Carlos Eduardo |
| author2_role | author author author author author author |
| author_facet | Zago, Chaiene Evelin Silva, Sónia Carina Sanitá, Paula Volpato Barbugli, Paula Aboud Dias, Carla Maria Improta Lordello, Virgínia Barreto Vergani, Carlos Eduardo |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Zago, Chaiene Evelin\"},{\"Person.name\":\"Silva, Sónia Carina\"},{\"Person.name\":\"Sanitá, Paula Volpato\"},{\"Person.name\":\"Barbugli, Paula Aboud\"},{\"Person.name\":\"Dias, Carla Maria Improta\"},{\"Person.name\":\"Lordello, Virgínia Barreto\"},{\"Person.name\":\"Vergani, Carlos Eduardo\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Zago, Chaiene Evelin Silva, Sónia Carina Sanitá, Paula Volpato Barbugli, Paula Aboud Dias, Carla Maria Improta Lordello, Virgínia Barreto Vergani, Carlos Eduardo |
| datacite.date.Accepted.fl_str_mv | 2015-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-05-07T11:36:47Z |
| datacite.date.embargoed.fl_str_mv | 2015-05-07T11:36:47Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| datacite.titles.title.fl_str_mv | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Zago, Chaiene Evelin Silva, Sónia Carina Sanitá, Paula Volpato Barbugli, Paula Aboud Dias, Carla Maria Improta Lordello, Virgínia Barreto Vergani, Carlos Eduardo |
| dc.date.Accepted.fl_str_mv | 2015-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-05-07T11:36:47Z |
| dc.date.embargoed.fl_str_mv | 2015-05-07T11:36:47Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/35078 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Public Library of Science |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| dc.title.fl_str_mv | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Polymicrobial biofilms are an understudied and a clinically relevant problem. This study evaluates the interaction between C. albicans, and methicillin- susceptible (MSSA) and resistant (MRSA) S. aureus growing in single- and dual-species biofilms. Single and dual species adhesion (90 min) and biofilms (12, 24, and 48 h) were evaluated by complementary methods: counting colony-forming units (CFU mL-1), XTT-reduction, and crystal violet staining (CV). The secretion of hydrolytic enzymes by the 48 h biofilms was also evaluated using fluorimetric kits. Scanning electron microscopy (SEM) was used to assess biofilm structure. The results from quantification assays were compared using two-way ANOVAs with Tukey post-hoc tests, while data from enzymatic activities were analyzed by one-way Welch-ANOVA followed by Games-Howell post hoc test ( = 0.05). C. albicans, MSSA and MRSA were able to adhere and to form biofilm in both single or mixed cultures. In general, all microorganisms in both growth conditions showed a gradual increase in the number of cells and metabolic activity over time, reaching peak values between 12 h and 48 h (<0.05). C. albicans single- and dual-biofilms had significantly higher total biomass values (<0.05) than single biofilms of bacteria. Except for single MRSA biofilms, all microorganisms in both growth conditions secreted proteinase and phospholipase-C. SEM images revealed extensive adherence of bacteria to hyphal elements of C. albicans. C. albicans, MSSA, and MRSA can co-exist in biofilms without antagonism and in an apparent synergistic effect, with bacteria cells preferentially associated to C. albicans hyphal forms. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/a05fcd49-22aa-4aa2-8e12-9d16e999baff/download |
| id | rum_b5a653ca391646e91c218e06da7bbb89 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/35078 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/35078 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Zago, Chaiene Evelin Silva, Sónia Carina Sanitá, Paula Volpato Barbugli, Paula Aboud Dias, Carla Maria Improta Lordello, Virgínia Barreto Vergani, Carlos Eduardo |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Public Library of Science |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engPublic Library of ScienceporPolymicrobial biofilms are an understudied and a clinically relevant problem. This study evaluates the interaction between C. albicans, and methicillin- susceptible (MSSA) and resistant (MRSA) S. aureus growing in single- and dual-species biofilms. Single and dual species adhesion (90 min) and biofilms (12, 24, and 48 h) were evaluated by complementary methods: counting colony-forming units (CFU mL-1), XTT-reduction, and crystal violet staining (CV). The secretion of hydrolytic enzymes by the 48 h biofilms was also evaluated using fluorimetric kits. Scanning electron microscopy (SEM) was used to assess biofilm structure. The results from quantification assays were compared using two-way ANOVAs with Tukey post-hoc tests, while data from enzymatic activities were analyzed by one-way Welch-ANOVA followed by Games-Howell post hoc test ( = 0.05). C. albicans, MSSA and MRSA were able to adhere and to form biofilm in both single or mixed cultures. In general, all microorganisms in both growth conditions showed a gradual increase in the number of cells and metabolic activity over time, reaching peak values between 12 h and 48 h (<0.05). C. albicans single- and dual-biofilms had significantly higher total biomass values (<0.05) than single biofilms of bacteria. Except for single MRSA biofilms, all microorganisms in both growth conditions secreted proteinase and phospholipase-C. SEM images revealed extensive adherence of bacteria to hyphal elements of C. albicans. C. albicans, MSSA, and MRSA can co-exist in biofilms without antagonism and in an apparent synergistic effect, with bacteria cells preferentially associated to C. albicans hyphal forms.application/pdfporDynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA)Zago, Chaiene EvelinSilva, Sónia CarinaSanitá, Paula VolpatoBarbugli, Paula AboudDias, Carla Maria ImprotaLordello, Virgínia BarretoVergani, Carlos EduardoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONZago, Chaiene Evelin; Silva, Sónia Carina; Sanitá, Paula Volpato; Barbugli, Paula Aboud; Dias, Carla Maria Improta; Lordello, Virgínia Barreto; Vergani, Carlos Eduardo, Dynamics of biofilm formation and the interaction between Candida albicans and Methicillin-susceptible (MSSA) and -Resistant Staphylococcus aureus (MRSA). PLoS One, 10(4), e0123206, 2015PMID25875834ISSNIsPartOf1932-6203EISSNIsPartOf1932-6203DOIIsPartOf10.1371/journal.pone.01232062015-05-07T11:36:47Z2015-042014-112015-05-06T10:45:43Z2015-04-01T00:00:00ZHandlehttps://hdl.handle.net/1822/35078http://purl.org/coar/access_right/c_abf2open accesshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Engenharia e Tecnologia::Engenharia Médicahttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Médicas::Biotecnologia Médica2473385 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/a05fcd49-22aa-4aa2-8e12-9d16e999baff/download |
| spellingShingle | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) Zago, Chaiene Evelin Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| status | SINGLETON |
| subject.other.fl_str_mv | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| title | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| title_full | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| title_fullStr | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| title_full_unstemmed | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| title_short | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| title_sort | Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA) |
| topic | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| topic_facet | Engenharia e Tecnologia::Engenharia Médica Ciências Médicas::Biotecnologia Médica |
| url | https://hdl.handle.net/1822/35078 |
| visible | 1 |