Publicação
VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis
| Resumo: | Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex region, that be used as a sex marker. Both genes are good candidates to be involved in flower sex differentiation in grapevine. To assess their role in sex specification, spatial and temporal expression analysis was performed. The expression of VviFSEX is detected in petals, stamens and carpel primordia of all flower types, making its putative function unclear; however, female plants display a single allele for this gene, while male and hermaphrodites display two alleles. On the other hand, the specific expression of VviAPRT3 in the carpel primordial of male plants suggests a possible role in the abortion of pistil structures. We propose a model to explain the carpel abortion in male flowers and the absence of stamen viability in female flowers. In addition, this work reinforces the presence of a sex locus on Vitis chromosome 2. |
|---|---|
| Autores principais: | Coito, João L. |
| Outros Autores: | Ramos, Miguel J. N.; Cunha, Jorge; Silva, Helena Sofia Gomes; Amâncio, Sara; Costa, Maria Manuela Ribeiro; Rocheta, Margarida |
| Assunto: | Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867437956773445632 |
|---|---|
| author | Coito, João L. |
| author2 | Ramos, Miguel J. N. Cunha, Jorge Silva, Helena Sofia Gomes Amâncio, Sara Costa, Maria Manuela Ribeiro Rocheta, Margarida |
| author2_role | author author author author author author |
| author_facet | Coito, João L. Ramos, Miguel J. N. Cunha, Jorge Silva, Helena Sofia Gomes Amâncio, Sara Costa, Maria Manuela Ribeiro Rocheta, Margarida |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Coito, João L.\"},{\"Person.name\":\"Ramos, Miguel J. N.\"},{\"Person.name\":\"Cunha, Jorge\"},{\"Person.name\":\"Silva, Helena Sofia Gomes\"},{\"Person.name\":\"Amâncio, Sara\"},{\"Person.name\":\"Costa, Maria Manuela Ribeiro\"},{\"Person.name\":\"Rocheta, Margarida\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Coito, João L. Ramos, Miguel J. N. Cunha, Jorge Silva, Helena Sofia Gomes Amâncio, Sara Costa, Maria Manuela Ribeiro Rocheta, Margarida |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-02-28T10:36:21Z |
| datacite.date.embargoed.fl_str_mv | 2018-02-28T10:36:21Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| datacite.titles.title.fl_str_mv | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Coito, João L. Ramos, Miguel J. N. Cunha, Jorge Silva, Helena Sofia Gomes Amâncio, Sara Costa, Maria Manuela Ribeiro Rocheta, Margarida |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-02-28T10:36:21Z |
| dc.date.embargoed.fl_str_mv | 2018-02-28T10:36:21Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/51192 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers Media |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| dc.title.fl_str_mv | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex region, that be used as a sex marker. Both genes are good candidates to be involved in flower sex differentiation in grapevine. To assess their role in sex specification, spatial and temporal expression analysis was performed. The expression of VviFSEX is detected in petals, stamens and carpel primordia of all flower types, making its putative function unclear; however, female plants display a single allele for this gene, while male and hermaphrodites display two alleles. On the other hand, the specific expression of VviAPRT3 in the carpel primordial of male plants suggests a possible role in the abortion of pistil structures. We propose a model to explain the carpel abortion in male flowers and the absence of stamen viability in female flowers. In addition, this work reinforces the presence of a sex locus on Vitis chromosome 2. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/e646c38b-f1b2-48ef-8b32-71c5e7b2158d/download |
| id | rum_e30940ef5fa03c2a1df8e10ec0ae51ee |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/51192 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/51192 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Coito, João L. Ramos, Miguel J. N. Cunha, Jorge Silva, Helena Sofia Gomes Amâncio, Sara Costa, Maria Manuela Ribeiro Rocheta, Margarida |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Frontiers Media |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engFrontiers MediaporVitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex region, that be used as a sex marker. Both genes are good candidates to be involved in flower sex differentiation in grapevine. To assess their role in sex specification, spatial and temporal expression analysis was performed. The expression of VviFSEX is detected in petals, stamens and carpel primordia of all flower types, making its putative function unclear; however, female plants display a single allele for this gene, while male and hermaphrodites display two alleles. On the other hand, the specific expression of VviAPRT3 in the carpel primordial of male plants suggests a possible role in the abortion of pistil structures. We propose a model to explain the carpel abortion in male flowers and the absence of stamen viability in female flowers. In addition, this work reinforces the presence of a sex locus on Vitis chromosome 2.application/pdfporVviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in VitisCoito, João L.Ramos, Miguel J. N.Cunha, JorgeSilva, Helena Sofia GomesAmâncio, SaraCosta, Maria Manuela RibeiroRocheta, MargaridaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONCoito, J. L., Ramos, M. J., Cunha, J., Silva, H. G., Amâncio, S., Costa, M. M., & Rocheta, M. (2017). VviAPRT3 and VviFSEX: Two genes involved in sex specification able to distinguish different flower types in Vitis. Frontiers in plant science, 8, 98ISSNIsPartOf1664-462XEISSNIsPartOf1664-462XDOIIsPartOf10.3389/fpls.2017.000982018-02-28T10:36:21Z20172017-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/51192http://purl.org/coar/access_right/c_abf2open accessVitisHermaphroditeDioeciousFlowerGene markerSexhttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Biológicas3976141 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/e646c38b-f1b2-48ef-8b32-71c5e7b2158d/download |
| spellingShingle | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis Coito, João L. Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| status | SINGLETON |
| subject.fl_str_mv | Vitis Hermaphrodite Dioecious Flower Gene marker Sex |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Biológicas |
| title | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| title_full | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| title_fullStr | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| title_full_unstemmed | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| title_short | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| title_sort | VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distinguish Different Flower Types in Vitis |
| topic | Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| topic_facet | Vitis Hermaphrodite Dioecious Flower Gene marker Sex Ciências Naturais::Ciências Biológicas |
| url | https://hdl.handle.net/1822/51192 |
| visible | 1 |