Publicação
Liposome formation with wool lipid extracts rich in ceramides
| Resumo: | Internal wool lipids (IWLs) are rich in cholesterol, free fatty acids, cholesteryl sulfate, and, mainly, ceramides. The repairing effect of these lipids structured as liposomes was demonstrated by reinforcing the skin-barrier integrity and increasing the water-holding capacity when applied onto the skin. This work was focused on the formation of liposomes with IWLs rich in ceramides, obtained at pilot plant level with organic solvent extraction by using methanol and acetone. The lipid composition of the two extracts was quantitatively analyzed. IWL extracts containing different amounts of sterol sulfate were used to form liposomes at physiologic pH. Vesicle size distribution, polydispersity index, and zeta potential of all liposomes were determined to characterize them and to study their stability. The results obtained showed that IWL extract composition, which was different depending on the extraction methodologies used, greatly influences the characteristics of the liposomes formed. Vesicular size and polydispersity index liposomes were smaller when the extract composition contained a higher proportion of either free fatty acids or sterol sulfate. Moreover, liposome stability was improved when some amount of sterol sulfate was added to the composition of methanol and acetone extracts. This natural mixture with keratinaceous origin could have a special interest for cosmetic or dermopharmaceutical companies. |
|---|---|
| Autores principais: | Ramírez, R. |
| Outros Autores: | Martí, M.; Paulo, Artur Cavaco; Silva, R.; de la Maza, A.; Parra, J. L.; Coderch, L. |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439560061878272 |
|---|---|
| author | Ramírez, R. |
| author2 | Martí, M. Paulo, Artur Cavaco Silva, R. de la Maza, A. Parra, J. L. Coderch, L. |
| author2_role | author author author author author author |
| author_facet | Ramírez, R. Martí, M. Paulo, Artur Cavaco Silva, R. de la Maza, A. Parra, J. L. Coderch, L. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ramírez, R.\"},{\"Person.name\":\"Martí, M.\"},{\"Person.name\":\"Paulo, Artur Cavaco\"},{\"Person.name\":\"Silva, R.\"},{\"Person.name\":\"de la Maza, A.\"},{\"Person.name\":\"Parra, J. L.\"},{\"Person.name\":\"Coderch, L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Ramírez, R. Martí, M. Paulo, Artur Cavaco Silva, R. de la Maza, A. Parra, J. L. Coderch, L. |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-02-21T17:03:30Z |
| datacite.date.embargoed.fl_str_mv | 2012-02-21T17:03:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Liposome formation with wool lipid extracts rich in ceramides |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Ramírez, R. Martí, M. Paulo, Artur Cavaco Silva, R. de la Maza, A. Parra, J. L. Coderch, L. |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-02-21T17:03:30Z |
| dc.date.embargoed.fl_str_mv | 2012-02-21T17:03:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/17308 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Informa Healthcare |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Liposome formation with wool lipid extracts rich in ceramides |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Internal wool lipids (IWLs) are rich in cholesterol, free fatty acids, cholesteryl sulfate, and, mainly, ceramides. The repairing effect of these lipids structured as liposomes was demonstrated by reinforcing the skin-barrier integrity and increasing the water-holding capacity when applied onto the skin. This work was focused on the formation of liposomes with IWLs rich in ceramides, obtained at pilot plant level with organic solvent extraction by using methanol and acetone. The lipid composition of the two extracts was quantitatively analyzed. IWL extracts containing different amounts of sterol sulfate were used to form liposomes at physiologic pH. Vesicle size distribution, polydispersity index, and zeta potential of all liposomes were determined to characterize them and to study their stability. The results obtained showed that IWL extract composition, which was different depending on the extraction methodologies used, greatly influences the characteristics of the liposomes formed. Vesicular size and polydispersity index liposomes were smaller when the extract composition contained a higher proportion of either free fatty acids or sterol sulfate. Moreover, liposome stability was improved when some amount of sterol sulfate was added to the composition of methanol and acetone extracts. This natural mixture with keratinaceous origin could have a special interest for cosmetic or dermopharmaceutical companies. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/b32246f9-036f-4be5-a5a0-4df4024db50e/download |
| id | rum_e6cffd1fe25cb2ebf6dfe9e02b1fefb5 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/17308 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/17308 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Ramírez, R. Martí, M. Paulo, Artur Cavaco Silva, R. de la Maza, A. Parra, J. L. Coderch, L. |
| publishDate | 2009 |
| publisher.none.fl_str_mv | Informa Healthcare |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engInforma HealthcareporInternal wool lipids (IWLs) are rich in cholesterol, free fatty acids, cholesteryl sulfate, and, mainly, ceramides. The repairing effect of these lipids structured as liposomes was demonstrated by reinforcing the skin-barrier integrity and increasing the water-holding capacity when applied onto the skin. This work was focused on the formation of liposomes with IWLs rich in ceramides, obtained at pilot plant level with organic solvent extraction by using methanol and acetone. The lipid composition of the two extracts was quantitatively analyzed. IWL extracts containing different amounts of sterol sulfate were used to form liposomes at physiologic pH. Vesicle size distribution, polydispersity index, and zeta potential of all liposomes were determined to characterize them and to study their stability. The results obtained showed that IWL extract composition, which was different depending on the extraction methodologies used, greatly influences the characteristics of the liposomes formed. Vesicular size and polydispersity index liposomes were smaller when the extract composition contained a higher proportion of either free fatty acids or sterol sulfate. Moreover, liposome stability was improved when some amount of sterol sulfate was added to the composition of methanol and acetone extracts. This natural mixture with keratinaceous origin could have a special interest for cosmetic or dermopharmaceutical companies.application/pdfporLiposome formation with wool lipid extracts rich in ceramidesRamírez, R.Martí, M.Paulo, Artur CavacoSilva, R.de la Maza, A.Parra, J. L.Coderch, L.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptPMID19515010ISSNIsPartOf0898-2104DOIIsPartOf10.1080/089821008025388382012-02-21T17:03:30Z20092009-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/17308http://purl.org/coar/access_right/c_abf2open access484292 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/b32246f9-036f-4be5-a5a0-4df4024db50e/download |
| spellingShingle | Liposome formation with wool lipid extracts rich in ceramides Ramírez, R. |
| status | SINGLETON |
| title | Liposome formation with wool lipid extracts rich in ceramides |
| title_full | Liposome formation with wool lipid extracts rich in ceramides |
| title_fullStr | Liposome formation with wool lipid extracts rich in ceramides |
| title_full_unstemmed | Liposome formation with wool lipid extracts rich in ceramides |
| title_short | Liposome formation with wool lipid extracts rich in ceramides |
| title_sort | Liposome formation with wool lipid extracts rich in ceramides |
| url | https://hdl.handle.net/1822/17308 |
| visible | 1 |