Publicação
Preparation and characterization of light-sensitive nanoparticles for cancer therapy
| Resumo: | Cancer is a significant health challenge to human life, which results in the development of innovative treatment approaches, such as photothermal therapy (PTT) and photodynamic therapy (PDT). PTT utilizes light-absorbing agents that can convert light into heat to selectively target and destroy the cancer cells through localized hyperthermia. Whereas PDT involves photosensitizers that generate reactive oxygen species (ROS) upon light activation to induce tumor cell death. This thesis aimed to develop polyethylenimine (PEI) / Gold (Au) / Manganese (Mn) based nanoparticles (PMA NPs) capable of combining different therapies, including light-based PTT, PDT, chemodynamic therapy, and chemotherapy. PMA NPS were optimized and characterized to produce spherical NPs with a hydrodynamic diameter (176.2 ± 1.78 nm) and a narrow size distribution, (0.069 ± 0.013), rendering them suitable for cancer treatment applications. The safety of PMA NPs was tested with different cell lines, up to a concentration of 300 μg/mL. Moreover, the nanosystem exhibited good photothermal conversion ability, which can cause temperature increase only upon 0.4 W laser irradiation, showing the potential of causing tumor cell damage or necrosis. Moreover, the incorporation of Indocyanine Green (ICG) into PMA NPs enhanced the generation of ROS and the photothermal effect with a 10.2 ºC temperature increase under the same power laser irradiation. Furthermore, with chemical conjugation of the functionalized paclitaxel (PTX) on the PMA NPs, PTX-L-PMA-ICG NPs were formed to be further used in cancer combination therapies. |
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| Autores principais: | Silva, Ana Catarina Rodrigues da |
| Assunto: | Nanoparticles Phototherapy Photodynamic therapy Chemotherapy Au NPs MnXOY NPs Polymeric NPs Cancer ICG Nanopartículas Fototerapia Terapia fotodinâmica Quimioterapia NPs de Au NPs de MnXOY NPs poliméricas Cancro Engenharia e Tecnologia::Engenharia Médica |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Cancer is a significant health challenge to human life, which results in the development of innovative treatment approaches, such as photothermal therapy (PTT) and photodynamic therapy (PDT). PTT utilizes light-absorbing agents that can convert light into heat to selectively target and destroy the cancer cells through localized hyperthermia. Whereas PDT involves photosensitizers that generate reactive oxygen species (ROS) upon light activation to induce tumor cell death. This thesis aimed to develop polyethylenimine (PEI) / Gold (Au) / Manganese (Mn) based nanoparticles (PMA NPs) capable of combining different therapies, including light-based PTT, PDT, chemodynamic therapy, and chemotherapy. PMA NPS were optimized and characterized to produce spherical NPs with a hydrodynamic diameter (176.2 ± 1.78 nm) and a narrow size distribution, (0.069 ± 0.013), rendering them suitable for cancer treatment applications. The safety of PMA NPs was tested with different cell lines, up to a concentration of 300 μg/mL. Moreover, the nanosystem exhibited good photothermal conversion ability, which can cause temperature increase only upon 0.4 W laser irradiation, showing the potential of causing tumor cell damage or necrosis. Moreover, the incorporation of Indocyanine Green (ICG) into PMA NPs enhanced the generation of ROS and the photothermal effect with a 10.2 ºC temperature increase under the same power laser irradiation. Furthermore, with chemical conjugation of the functionalized paclitaxel (PTX) on the PMA NPs, PTX-L-PMA-ICG NPs were formed to be further used in cancer combination therapies. |
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