Publicação
Biological Techniques
| Resumo: | Gamma irradiation causes several types of biological changes in foods and food ingredients subjected to this technology. DNA is the major cellular target of ionizing radiation, and the resulting damage is responsible for the inactivation of microorganisms, inhibition of growth, and other degradative effects. Hence, analytical methods for the molecular damage to the DNA of foods themselves or of microorganisms usually associated, for the microbial load and diversity, and for the ability to germinate are being developed and applied as rapid, simple, and inexpensive procedures for screening irradiated foods, either qualitatively or for the estimation of the applied dose. The most commonly used biological methods for the detection of irradiated foods are Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC), the DNA comet assay, and the Limulus Amebocyte Lysate (LAL) test. These are currently standardized methods, but others are under development. Since biological changes are generally not radiation-specific, the biological methods detailed in this chapter are typically used for screening, and should subsequently be complemented by confirmative chemical or physical methods, such as the standard methods described in Chapters 13 and 14. |
|---|---|
| Autores principais: | Rodrigues, Paula |
| Outros Autores: | Venâncio, Armando |
| Assunto: | Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | capítulo de livro |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439433145384960 |
|---|---|
| author | Rodrigues, Paula |
| author2 | Venâncio, Armando |
| author2_role | author |
| author_facet | Rodrigues, Paula Venâncio, Armando |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Rodrigues, Paula\"},{\"Person.name\":\"Venâncio, Armando\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Paula Venâncio, Armando |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| datacite.titles.title.fl_str_mv | Biological Techniques |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Rodrigues, Paula Venâncio, Armando |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/55749 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| dc.title.fl_str_mv | Biological Techniques |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_3248 |
| description | Gamma irradiation causes several types of biological changes in foods and food ingredients subjected to this technology. DNA is the major cellular target of ionizing radiation, and the resulting damage is responsible for the inactivation of microorganisms, inhibition of growth, and other degradative effects. Hence, analytical methods for the molecular damage to the DNA of foods themselves or of microorganisms usually associated, for the microbial load and diversity, and for the ability to germinate are being developed and applied as rapid, simple, and inexpensive procedures for screening irradiated foods, either qualitatively or for the estimation of the applied dose. The most commonly used biological methods for the detection of irradiated foods are Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC), the DNA comet assay, and the Limulus Amebocyte Lysate (LAL) test. These are currently standardized methods, but others are under development. Since biological changes are generally not radiation-specific, the biological methods detailed in this chapter are typically used for screening, and should subsequently be complemented by confirmative chemical or physical methods, such as the standard methods described in Chapters 13 and 14. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | bookPart |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/a30cb902-4c07-4b2c-8cff-95a4f4f69d79/download |
| id | rum_e8a6d7ea8e5bb62d0f882b508f207160 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/55749 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
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| network_name_str | RepositóriUM - Universidade do Minho |
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| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Rodrigues, Paula Venâncio, Armando |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engRoyal Society of ChemistryporGamma irradiation causes several types of biological changes in foods and food ingredients subjected to this technology. DNA is the major cellular target of ionizing radiation, and the resulting damage is responsible for the inactivation of microorganisms, inhibition of growth, and other degradative effects. Hence, analytical methods for the molecular damage to the DNA of foods themselves or of microorganisms usually associated, for the microbial load and diversity, and for the ability to germinate are being developed and applied as rapid, simple, and inexpensive procedures for screening irradiated foods, either qualitatively or for the estimation of the applied dose. The most commonly used biological methods for the detection of irradiated foods are Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC), the DNA comet assay, and the Limulus Amebocyte Lysate (LAL) test. These are currently standardized methods, but others are under development. Since biological changes are generally not radiation-specific, the biological methods detailed in this chapter are typically used for screening, and should subsequently be complemented by confirmative chemical or physical methods, such as the standard methods described in Chapters 13 and 14.application/pdfporBiological TechniquesRodrigues, PaulaVenâncio, ArmandoHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISBNIsPartOf978-1-78262-708-1ISSNIsPartOf2398-0656DOIIsPartOf10.1039/9781788010252-00314EISBNIsPartOf978-1-78801-025-220182018-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/55749http://purl.org/coar/access_right/c_16ecrestricted accessIrradiaçãoAlimentoshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Engenharia e Tecnologia::Biotecnologia Industrial122741 bytesliteraturehttp://purl.org/coar/resource_type/c_3248book parthttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorium.uminho.pt/bitstreams/a30cb902-4c07-4b2c-8cff-95a4f4f69d79/download |
| spellingShingle | Biological Techniques Rodrigues, Paula Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| status | SINGLETON |
| subject.fl_str_mv | Irradiação Alimentos |
| subject.other.fl_str_mv | Engenharia e Tecnologia::Biotecnologia Industrial |
| title | Biological Techniques |
| title_full | Biological Techniques |
| title_fullStr | Biological Techniques |
| title_full_unstemmed | Biological Techniques |
| title_short | Biological Techniques |
| title_sort | Biological Techniques |
| topic | Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| topic_facet | Irradiação Alimentos Engenharia e Tecnologia::Biotecnologia Industrial |
| url | https://hdl.handle.net/1822/55749 |
| visible | 1 |