Publicação

Functionalized microparticles producing scaffolds in combination with cells

Detalhes bibliográficos
Resumo:	The development of biologically instructive biomaterials with application for tissue regeneration has become the focus of intense research over the last years. This work reports a novel approach for the production of three-dimensional constructs for tissue engineering applications based on the assembly of chitosan microparticles exhibiting specific biological response with cells. Chitosan microparticles with a size range between 20 and 70 μm are functionalized with platelet derived growth factor (PDFG-BB). The functionalization is achieved by previous immobilization of an anti-PDGF-BB antibody, using a water-soluble carbodiimide. When incubated with a cocktail of growth factors-platelet lysates, the previously functionalized particles are able to target PDGF-BB from the protein mixture. In vitro studies are carried out focusing on the ability of these systems to promote the assembly into a stable 3D construct triggered by the presence of human adipose stem cells, which act as crosslinker agents and induce the formation of a hydrogel network. The presence of immobilized growth factors gives to this system a biological functionality towards control on cell function. It is also bioresponsive, as cells drive the assembly process of the microgel. These versatile biomimetic microgels may provide a powerful tool to be used as an injectable system for non-invasive tissue engineering applications with additional control over cellular function by creating specific microenvironments for cell growth.
Autores principais:	Custódio, Catarina A.
Outros Autores:	Santo, Vitor E.; Oliveira, Mariana B.; Gomes, Manuela E.; Reis, R. L.; Mano, J. F.
Assunto:	cell microenvironments Chitosan microparticles regenerative medicine microscale engineering functionalization platelet lysates
Ano:	2014
País:	Portugal
Tipo de documento:	artigo
Tipo de acesso:	acesso restrito
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	The development of biologically instructive biomaterials with application for tissue regeneration has become the focus of intense research over the last years. This work reports a novel approach for the production of three-dimensional constructs for tissue engineering applications based on the assembly of chitosan microparticles exhibiting specific biological response with cells. Chitosan microparticles with a size range between 20 and 70 μm are functionalized with platelet derived growth factor (PDFG-BB). The functionalization is achieved by previous immobilization of an anti-PDGF-BB antibody, using a water-soluble carbodiimide. When incubated with a cocktail of growth factors-platelet lysates, the previously functionalized particles are able to target PDGF-BB from the protein mixture. In vitro studies are carried out focusing on the ability of these systems to promote the assembly into a stable 3D construct triggered by the presence of human adipose stem cells, which act as crosslinker agents and induce the formation of a hydrogel network. The presence of immobilized growth factors gives to this system a biological functionality towards control on cell function. It is also bioresponsive, as cells drive the assembly process of the microgel. These versatile biomimetic microgels may provide a powerful tool to be used as an injectable system for non-invasive tissue engineering applications with additional control over cellular function by creating specific microenvironments for cell growth.