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Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

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Resumo:The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
Autores principais:Ferreira, Sílvia A.
Outros Autores:Correia, Alexandra; Madureira, Pedro; Vilanova, Manuel; Gama, F. M.
Assunto:Nanogel Cell internalization Confocal microscopy NanogelCell endocytosis imaging mannan nanogel self-assembly
Ano:2012
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho

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