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Cutinase: a new tool for biomodification of synthetic fibers

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Detalhes bibliográficos
Resumo:This paper describes two methods to monitor esterase hydrolysis at the surface of polyester fibres (PETPolyethylene terephthalate). The hydroxyl groups were determined on the fibre surface by alkaline reaction with a reactive dye (CI Reactive Black 5) and colour intensity was determined using a reflectance spectrophotometer. The terephthalic acid solution formed was also quantified after reaction with peroxide by fluorimetric determination of the resulting hydroxyterephthalic acid. Detailed descriptions of those methods are given in this paper.
Autores principais:Silva, Carla Manuela Pereira Marinho da
Outros Autores:Carneiro, Ana Filipa Gonçalves da Costa; O'Neill, Jaime Alexandre Antunes; Fonseca, Luís P.; Cabral, J. M. S.; Gübitz, Georg M.; Paulo, Artur Cavaco
Assunto:Acrylic Amidase Cutinase Esterase Polyamide 6.6 Polyester
Ano:2005
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:This paper describes two methods to monitor esterase hydrolysis at the surface of polyester fibres (PETPolyethylene terephthalate). The hydroxyl groups were determined on the fibre surface by alkaline reaction with a reactive dye (CI Reactive Black 5) and colour intensity was determined using a reflectance spectrophotometer. The terephthalic acid solution formed was also quantified after reaction with peroxide by fluorimetric determination of the resulting hydroxyterephthalic acid. Detailed descriptions of those methods are given in this paper.