Publicação
The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity
| Resumo: | Bacteria are believed to play an important role in the fitness and biochemistry of sponges (Porifera). Pseudomonas species (Gammaproteobacteria, Pseudomonadales) are capable of colonizing a broad range of eukaryotic hosts, but knowledge of their diversity and function in freshwater invertebrates is rudimentary. We assessed the diversity, structure and antimicrobial activities of Pseudomonas spp. in the freshwater sponge Ephydatia fluviatilis. Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprints of the global regulator gene gacA revealed distinct structures between sponge-associated and free-living Pseudomonas communities, unveiling previously unsuspected diversity of these assemblages in freshwater. Community structures varied across E. fluviatilis specimens, yet specific gacA phylotypes could be detected by PCR-DGGE in almost all sponge individuals sampled over two consecutive years. By means of whole-genome fingerprinting, 39 distinct genotypes were found within 90 fluorescent Pseudomonas isolates retrieved from E. fluviatilis. High frequency of in vitro antibacterial (49%), antiprotozoan (35%) and anti-oomycetal (32%) activities was found among these isolates, contrasting less-pronounced basidiomycetal (17%) and ascomycetal (8%) antagonism. Culture extracts of highly predation-resistant isolates rapidly caused complete immobility or lysis of cells of the protozoan Colpoda steinii. Isolates tentatively identified as P. jessenii, P. protegens and P. oryzihabitans showed conspicuous inhibitory traits and correspondence with dominant sponge-associated phylotypes registered by cultivation-independent analysis. Our findings suggest that E. fluviatilis hosts both transient and persistent Pseudomonas symbionts displaying antimicrobial activities of potential ecological and biotechnological value. |
|---|---|
| Autores principais: | Keller-Costa, Tina |
| Outros Autores: | Jousset, Alexandre; van Overbeek, Leo; van Elsas, Jan Dirk; Costa, Rodrigo |
| Assunto: | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Algarve |
| Idioma: | inglês |
| Origem: | Sapientia - Universidade do Algarve |
| _version_ | 1867050995590103040 |
|---|---|
| author | Keller-Costa, Tina |
| author2 | Jousset, Alexandre van Overbeek, Leo van Elsas, Jan Dirk Costa, Rodrigo |
| author2_role | author author author author |
| author_facet | Keller-Costa, Tina Jousset, Alexandre van Overbeek, Leo van Elsas, Jan Dirk Costa, Rodrigo |
| author_role | author |
| contributor_name_str_mv | Sapientia |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Keller-Costa, Tina\",\"Person.identifier.orcid\":\"0000-0003-3702-9192\"},{\"Person.name\":\"Jousset, Alexandre\"},{\"Person.name\":\"van Overbeek, Leo\"},{\"Person.name\":\"van Elsas, Jan Dirk\"},{\"Person.name\":\"Costa, Rodrigo\",\"Person.identifier.orcid\":\"0000-0002-5932-4101\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Sapientia |
| datacite.creators.creator.creatorName.fl_str_mv | Keller-Costa, Tina Jousset, Alexandre van Overbeek, Leo van Elsas, Jan Dirk Costa, Rodrigo |
| datacite.date.Accepted.fl_str_mv | 2014-02-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-12-07T14:53:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-12-07T14:53:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| datacite.titles.title.fl_str_mv | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| dc.contributor.none.fl_str_mv | Sapientia |
| dc.creator.none.fl_str_mv | Keller-Costa, Tina Jousset, Alexandre van Overbeek, Leo van Elsas, Jan Dirk Costa, Rodrigo |
| dc.date.Accepted.fl_str_mv | 2014-02-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-12-07T14:53:00Z |
| dc.date.embargoed.fl_str_mv | 2018-12-07T14:53:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.1/11310 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Public Library of Science |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| dc.title.fl_str_mv | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bacteria are believed to play an important role in the fitness and biochemistry of sponges (Porifera). Pseudomonas species (Gammaproteobacteria, Pseudomonadales) are capable of colonizing a broad range of eukaryotic hosts, but knowledge of their diversity and function in freshwater invertebrates is rudimentary. We assessed the diversity, structure and antimicrobial activities of Pseudomonas spp. in the freshwater sponge Ephydatia fluviatilis. Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprints of the global regulator gene gacA revealed distinct structures between sponge-associated and free-living Pseudomonas communities, unveiling previously unsuspected diversity of these assemblages in freshwater. Community structures varied across E. fluviatilis specimens, yet specific gacA phylotypes could be detected by PCR-DGGE in almost all sponge individuals sampled over two consecutive years. By means of whole-genome fingerprinting, 39 distinct genotypes were found within 90 fluorescent Pseudomonas isolates retrieved from E. fluviatilis. High frequency of in vitro antibacterial (49%), antiprotozoan (35%) and anti-oomycetal (32%) activities was found among these isolates, contrasting less-pronounced basidiomycetal (17%) and ascomycetal (8%) antagonism. Culture extracts of highly predation-resistant isolates rapidly caused complete immobility or lysis of cells of the protozoan Colpoda steinii. Isolates tentatively identified as P. jessenii, P. protegens and P. oryzihabitans showed conspicuous inhibitory traits and correspondence with dominant sponge-associated phylotypes registered by cultivation-independent analysis. Our findings suggest that E. fluviatilis hosts both transient and persistent Pseudomonas symbionts displaying antimicrobial activities of potential ecological and biotechnological value. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://sapientia.ualg.pt/bitstreams/a95cad03-2fc3-4763-b4b9-a1f28c79a5bb/download |
| id | sapientia_f19b167df0e4fdbb2c82e4eefdfdef62 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.1/11310 |
| instacron_str | ualg |
| institution | Universidade do Algarve |
| instname_str | Universidade do Algarve |
| language | eng |
| network_acronym_str | sapientia |
| network_name_str | Sapientia - Universidade do Algarve |
| oai_identifier_str | oai:sapientia.ualg.pt:10400.1/11310 |
| organization_str_mv | urn:organizationAcronym:ualg |
| person_str_mv | Keller-Costa, Tina Keller-Costa, Tina https://www.ciencia-id.pt/9319-A7D5-5335 9319-A7D5-5335 http://orcid.org/0000-0003-3702-9192 0000-0003-3702-9192 Jousset, Alexandre van Overbeek, Leo van Elsas, Jan Dirk Costa, Rodrigo Costa, Rodrigo https://www.ciencia-id.pt/5917-D500-D251 5917-D500-D251 http://orcid.org/0000-0002-5932-4101 0000-0002-5932-4101 |
| publishDate | 2014 |
| publisher.none.fl_str_mv | Public Library of Science |
| reponame_str | Sapientia - Universidade do Algarve |
| repository_id_str | urn:repositoryAcronym:sapientia |
| service_str_mv | urn:repositoryAcronym:sapientia |
| spelling | engPublic Library of ScienceBacteria are believed to play an important role in the fitness and biochemistry of sponges (Porifera). Pseudomonas species (Gammaproteobacteria, Pseudomonadales) are capable of colonizing a broad range of eukaryotic hosts, but knowledge of their diversity and function in freshwater invertebrates is rudimentary. We assessed the diversity, structure and antimicrobial activities of Pseudomonas spp. in the freshwater sponge Ephydatia fluviatilis. Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprints of the global regulator gene gacA revealed distinct structures between sponge-associated and free-living Pseudomonas communities, unveiling previously unsuspected diversity of these assemblages in freshwater. Community structures varied across E. fluviatilis specimens, yet specific gacA phylotypes could be detected by PCR-DGGE in almost all sponge individuals sampled over two consecutive years. By means of whole-genome fingerprinting, 39 distinct genotypes were found within 90 fluorescent Pseudomonas isolates retrieved from E. fluviatilis. High frequency of in vitro antibacterial (49%), antiprotozoan (35%) and anti-oomycetal (32%) activities was found among these isolates, contrasting less-pronounced basidiomycetal (17%) and ascomycetal (8%) antagonism. Culture extracts of highly predation-resistant isolates rapidly caused complete immobility or lysis of cells of the protozoan Colpoda steinii. Isolates tentatively identified as P. jessenii, P. protegens and P. oryzihabitans showed conspicuous inhibitory traits and correspondence with dominant sponge-associated phylotypes registered by cultivation-independent analysis. Our findings suggest that E. fluviatilis hosts both transient and persistent Pseudomonas symbionts displaying antimicrobial activities of potential ecological and biotechnological value.application/pdfThe freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activityPersonalKeller-Costa, TinaDSpacehttp://dspace.org/items/e5a30366-3797-42ff-944b-f29ba4136cbdDSpacehttp://dspace.org/items/e5a30366-3797-42ff-944b-f29ba4136cbdKeller-CostaTinaCiência IDhttps://www.ciencia-id.pt9319-A7D5-5335ORCIDhttp://orcid.org0000-0003-3702-9192Jousset, Alexandrevan Overbeek, Leovan Elsas, Jan DirkPersonalCosta, RodrigoDSpacehttp://dspace.org/items/4495127c-16f2-4231-9fc1-b471c661036bDSpacehttp://dspace.org/items/4495127c-16f2-4231-9fc1-b471c661036bda Silva CostaRodrigoCiência IDhttps://www.ciencia-id.pt5917-D500-D251ORCIDhttp://orcid.org0000-0002-5932-4101Researcher IDhttps://www.researcherid.comN-7274-2013Scopus Author IDhttps://www.scopus.com7203063627Scopus Author IDhttps://www.scopus.com55981105500HostingInstitutionOrganizationalSapientiae-mailmailto:repositorio@ualg.ptrepositorio@ualg.ptISSNIsPartOf1932-6203DOIIsPartOf10.1371/journal.pone.00884292018-12-07T14:53:00Z2014-022014-02-01T00:00:00ZHandlehttp://hdl.handle.net/10400.1/11310http://purl.org/coar/access_right/c_abf2open accessFluorescent pseudomonadsSecondary metabolitesPhenotypic variationBacterial symbiontBiological-controlBiofilm formationSmall RnasSp Nov.RhizosphereCommunity2979279 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2014-02http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://sapientia.ualg.pt/bitstreams/a95cad03-2fc3-4763-b4b9-a1f28c79a5bb/downloadPLoS ONE92e88429 |
| spellingShingle | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity Keller-Costa, Tina Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| status | SINGLETON |
| subject.fl_str_mv | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| title | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| title_full | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| title_fullStr | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| title_full_unstemmed | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| title_short | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| title_sort | The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity |
| topic | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| topic_facet | Fluorescent pseudomonads Secondary metabolites Phenotypic variation Bacterial symbiont Biological-control Biofilm formation Small Rnas Sp Nov. Rhizosphere Community |
| url | http://hdl.handle.net/10400.1/11310 |
| visible | 1 |