Publicação
Relationship between calcium in atherosclerotic plaques and bone mineral density : a clinical and histological perspective
| Resumo: | The relationship between osteoporosis (OP) and atherosclerosis is still unclear. Despite the epidemiological association and the existence of common risk factors such as the influence of age in both pathologies, there are clinical, genetic and pathogenic data, suggesting the possibility of a common pathway. Vascular calcification has been an important target of investigation. There are several studies that demonstrate increased vascular calcifications in patients with OP. The present study aimed to histologically evaluate the calcium deposits in atherosclerotic plaques of patients with and without osteoporosis, through a semi-quantitative analysis of calcium deposits. In this study were included 32 patients undergoing elective carotid endarterectomy, from which were obtained blood samples and the atheroma plaque. A dual-energy X-ray absorptiometry (DXA) was performed and a structured clinical protocol was applied. Samples were stained with Alizarin Red S and scanned with NanoZoomer®, in order to observe the calcium deposits within the atherosclerotic plaques. The semiquantitative analysis by ImageJ® showed numerically higher median values of relative calcium area in the atheroma plaques from osteoporotic patients (median: 45.46%) comparing to patients with normal bone mineral density (median: 15.73%), although this difference was not statistically significant (p-value = 0.175). In multivariate analysis, we do not found an independent relationship, between the relative area of calcium in atherosclerotic plaques with serum HDL levels and statins therapy, in the presence of osteoporosis, adjusted to gender and age. |
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| Autores principais: | Cruz, Rafael António Moiteiro da, 1992- |
| Assunto: | Aterosclerose Osteoporose Calcificação vascular Cálcio Reumatologia |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The relationship between osteoporosis (OP) and atherosclerosis is still unclear. Despite the epidemiological association and the existence of common risk factors such as the influence of age in both pathologies, there are clinical, genetic and pathogenic data, suggesting the possibility of a common pathway. Vascular calcification has been an important target of investigation. There are several studies that demonstrate increased vascular calcifications in patients with OP. The present study aimed to histologically evaluate the calcium deposits in atherosclerotic plaques of patients with and without osteoporosis, through a semi-quantitative analysis of calcium deposits. In this study were included 32 patients undergoing elective carotid endarterectomy, from which were obtained blood samples and the atheroma plaque. A dual-energy X-ray absorptiometry (DXA) was performed and a structured clinical protocol was applied. Samples were stained with Alizarin Red S and scanned with NanoZoomer®, in order to observe the calcium deposits within the atherosclerotic plaques. The semiquantitative analysis by ImageJ® showed numerically higher median values of relative calcium area in the atheroma plaques from osteoporotic patients (median: 45.46%) comparing to patients with normal bone mineral density (median: 15.73%), although this difference was not statistically significant (p-value = 0.175). In multivariate analysis, we do not found an independent relationship, between the relative area of calcium in atherosclerotic plaques with serum HDL levels and statins therapy, in the presence of osteoporosis, adjusted to gender and age. |
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