Publicação
Spectroscopic analysis of freeze-dried bacteria containing lozenges
| Resumo: | The aim of this work is to analyse a number of throat lozenges containing bacteria (Streptococcus salivarius K12). By the use of Raman and Infrared spectroscopies and Differential Scanning Calorimetry this study intends to evaluate the crystalline structure of the components in the lozenges. Moreover it would be interesting to know if the crystallinity and viability of the lozenges change as a function of storage or production parameters, hence an accelerated stability test has been performed. The lozenges in this study are the BLIS K12™, a probiotic for the mouth, ear, nose and throat that produces Bacteriocin-Like Inhibitory Substances (BLIS). Accelerated stability tests were performed during nine days in the following controlled conditions: 25°C, with 11% and 75% relative humidity; 40°C, with 11% and 75% relative humidity. Principal Component Analysis was performed for the IR and Raman spectroscopies and it revealed that the crystalline structure of the lozenges was retained. This work was successful in elaborating a suitable accelerated stability test. The techniques used were adequate to assess the condition of the lozenges during the course of the test. Lozenges appear to be very stable, since the results showed the crystalline structure of the lozenges remained intact. However the organoleptic properties did change towards the end of the stability test, decreasing their viability. The use of traditional Raman versus the new time resolved Raman, it is clear that the latter is an excellent technique to overcome the fluorescence problems the previous Raman possessed. |
|---|---|
| Autores principais: | Alves, Carolina de Sousa |
| Assunto: | Accelerated stability test BLIS Raman spectroscopy Streptococcus salivarius K12 Throat lozenges Mestrado Integrado - 2016 |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The aim of this work is to analyse a number of throat lozenges containing bacteria (Streptococcus salivarius K12). By the use of Raman and Infrared spectroscopies and Differential Scanning Calorimetry this study intends to evaluate the crystalline structure of the components in the lozenges. Moreover it would be interesting to know if the crystallinity and viability of the lozenges change as a function of storage or production parameters, hence an accelerated stability test has been performed. The lozenges in this study are the BLIS K12™, a probiotic for the mouth, ear, nose and throat that produces Bacteriocin-Like Inhibitory Substances (BLIS). Accelerated stability tests were performed during nine days in the following controlled conditions: 25°C, with 11% and 75% relative humidity; 40°C, with 11% and 75% relative humidity. Principal Component Analysis was performed for the IR and Raman spectroscopies and it revealed that the crystalline structure of the lozenges was retained. This work was successful in elaborating a suitable accelerated stability test. The techniques used were adequate to assess the condition of the lozenges during the course of the test. Lozenges appear to be very stable, since the results showed the crystalline structure of the lozenges remained intact. However the organoleptic properties did change towards the end of the stability test, decreasing their viability. The use of traditional Raman versus the new time resolved Raman, it is clear that the latter is an excellent technique to overcome the fluorescence problems the previous Raman possessed. |
|---|