Publicação
The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
| Resumo: | ABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future. |
|---|---|
| Autores principais: | Amaral, Ana |
| Outros Autores: | Fernandes, Carina; Szostek-Mioduchowska, Anna; Lukasik, Karolina; Rebordão, Maria Rosa; Pinto-Bravo, Pedro; Skarzynski, Dariusz Jan; Ferreira-Dias, Graça |
| Assunto: | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866809602262171648 |
|---|---|
| author | Amaral, Ana |
| author2 | Fernandes, Carina Szostek-Mioduchowska, Anna Lukasik, Karolina Rebordão, Maria Rosa Pinto-Bravo, Pedro Skarzynski, Dariusz Jan Ferreira-Dias, Graça |
| author2_role | author author author author author author author |
| author_facet | Amaral, Ana Fernandes, Carina Szostek-Mioduchowska, Anna Lukasik, Karolina Rebordão, Maria Rosa Pinto-Bravo, Pedro Skarzynski, Dariusz Jan Ferreira-Dias, Graça |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Amaral, Ana\",\"Person.identifier.orcid\":\"0000-0002-0075-262X\"},{\"Person.name\":\"Fernandes, Carina\"},{\"Person.name\":\"Szostek-Mioduchowska, Anna\"},{\"Person.name\":\"Lukasik, Karolina\"},{\"Person.name\":\"Rebordão, Maria Rosa\",\"Person.identifier.orcid\":\"0000-0002-4389-445X\"},{\"Person.name\":\"Pinto-Bravo, Pedro\"},{\"Person.name\":\"Skarzynski, Dariusz Jan\"},{\"Person.name\":\"Ferreira-Dias, Graça\",\"Person.identifier.orcid\":\"0000-0003-0622-6513\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Amaral, Ana Fernandes, Carina Szostek-Mioduchowska, Anna Lukasik, Karolina Rebordão, Maria Rosa Pinto-Bravo, Pedro Skarzynski, Dariusz Jan Ferreira-Dias, Graça |
| datacite.date.Accepted.fl_str_mv | 2021-10-19T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-17T14:52:03Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-17T14:52:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| datacite.titles.title.fl_str_mv | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Amaral, Ana Fernandes, Carina Szostek-Mioduchowska, Anna Lukasik, Karolina Rebordão, Maria Rosa Pinto-Bravo, Pedro Skarzynski, Dariusz Jan Ferreira-Dias, Graça |
| dc.date.Accepted.fl_str_mv | 2021-10-19T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-17T14:52:03Z |
| dc.date.embargoed.fl_str_mv | 2022-01-17T14:52:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/23107 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| dc.title.fl_str_mv | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | ABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/31e29aa1-fd1d-4cdc-9c69-41d2bd61b971/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ul_506b3f8726c989759d37dffdf7fc180a |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/23107 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/23107 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Amaral, Ana Amaral, Ana https://www.ciencia-id.pt/EB18-9420-421B EB18-9420-421B http://orcid.org/0000-0002-0075-262X 0000-0002-0075-262X Fernandes, Carina Szostek-Mioduchowska, Anna Lukasik, Karolina Rebordão, Maria Rosa Rebordão, Maria Rosa https://www.ciencia-id.pt/9515-257A-B35D 9515-257A-B35D http://orcid.org/0000-0002-4389-445X 0000-0002-4389-445X Pinto-Bravo, Pedro Skarzynski, Dariusz Jan Ferreira-Dias, Graça Ferreira-Dias, Graça https://www.ciencia-id.pt/F51C-7F45-C720 F51C-7F45-C720 http://orcid.org/0000-0003-0622-6513 0000-0003-0622-6513 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.application/pdfpt_PTThe inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometriumPersonalAmaral, AnaDSpacehttp://dspace.org/items/64ab1595-75c6-4b47-b196-ae1d494e6ca1DSpacehttp://dspace.org/items/64ab1595-75c6-4b47-b196-ae1d494e6ca1AmaralAnaCiência IDhttps://www.ciencia-id.ptEB18-9420-421BORCIDhttp://orcid.org0000-0002-0075-262XScopus Author IDhttps://www.scopus.com56394000500Fernandes, CarinaSzostek-Mioduchowska, AnnaLukasik, KarolinaPersonalRebordão, Maria RosaDSpacehttp://dspace.org/items/d1fd322a-70b1-4446-afe0-a5a4b9d1f5faDSpacehttp://dspace.org/items/d1fd322a-70b1-4446-afe0-a5a4b9d1f5faRebordão Cordeiro Simões CrisóstomoMaria RosaCiência IDhttps://www.ciencia-id.pt9515-257A-B35DORCIDhttp://orcid.org0000-0002-4389-445XPinto-Bravo, PedroSkarzynski, Dariusz JanPersonalFerreira-Dias, GraçaDSpacehttp://dspace.org/items/0fb8ec33-7ee1-482b-bdcb-eff773226f90DSpacehttp://dspace.org/items/0fb8ec33-7ee1-482b-bdcb-eff773226f90Leitão Ferreira-DiasGraça MariaCiência IDhttps://www.ciencia-id.ptF51C-7F45-C720ORCIDhttp://orcid.org0000-0003-0622-6513HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3390/life111011072022-01-17T14:52:03Z2021-10-192021-10-19T00:00:00ZHandlehttp://hdl.handle.net/10400.5/23107http://purl.org/coar/access_right/c_abf2open accessEquineEndometrosisFibrosisCollagenCathepsin GNoscapineInhibition2353535 bytesFundação para a Ciência e a TecnologiaCentre for Interdisciplinary Research in Animal Health6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosisCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2021-10-19http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/31e29aa1-fd1d-4cdc-9c69-41d2bd61b971/downloadLifev.11(10):1107Basel, Switzerland |
| spellingShingle | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium Amaral, Ana Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| status | SINGLETON |
| subject.fl_str_mv | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| title | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| title_full | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| title_fullStr | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| title_full_unstemmed | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| title_short | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| title_sort | The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium |
| topic | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| topic_facet | Equine Endometrosis Fibrosis Collagen Cathepsin G Noscapine Inhibition |
| url | http://hdl.handle.net/10400.5/23107 |
| visible | 1 |