Publicação
Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
| Resumo: | Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells. |
|---|---|
| Autores principais: | Cal, Pedro M.S.D. |
| Outros Autores: | Sieglitz, Florian; Santos, Fábio; Parente Carvalho, Cátia; Guerreiro, Ana; Bertoldo, Jean B.; Pischel, Uwe; Gois, Pedro M. P.; Bernardes, Gonçalo J. L. |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866809540269309952 |
|---|---|
| author | Cal, Pedro M.S.D. |
| author2 | Sieglitz, Florian Santos, Fábio Parente Carvalho, Cátia Guerreiro, Ana Bertoldo, Jean B. Pischel, Uwe Gois, Pedro M. P. Bernardes, Gonçalo J. L. |
| author2_role | author author author author author author author author |
| author_facet | Cal, Pedro M.S.D. Sieglitz, Florian Santos, Fábio Parente Carvalho, Cátia Guerreiro, Ana Bertoldo, Jean B. Pischel, Uwe Gois, Pedro M. P. Bernardes, Gonçalo J. L. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cal, Pedro M.S.D.\",\"Person.identifier.orcid\":\"0000-0002-2512-7038\"},{\"Person.name\":\"Sieglitz, Florian\",\"Person.identifier.orcid\":\"0000-0003-4480-8450\"},{\"Person.name\":\"Santos, Fábio\",\"Person.identifier.orcid\":\"0000-0002-9057-9276\"},{\"Person.name\":\"Parente Carvalho, Cátia\"},{\"Person.name\":\"Guerreiro, Ana\",\"Person.identifier.orcid\":\"0000-0002-2173-6514\"},{\"Person.name\":\"Bertoldo, Jean B.\"},{\"Person.name\":\"Pischel, Uwe\"},{\"Person.name\":\"Gois, Pedro M. P.\",\"Person.identifier.orcid\":\"0000-0002-7698-630X\"},{\"Person.name\":\"Bernardes, Gonçalo J. L.\",\"Person.identifier.orcid\":\"0000-0001-6594-8917\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Cal, Pedro M.S.D. Sieglitz, Florian Santos, Fábio Parente Carvalho, Cátia Guerreiro, Ana Bertoldo, Jean B. Pischel, Uwe Gois, Pedro M. P. Bernardes, Gonçalo J. L. |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-02-21T18:00:39Z |
| datacite.date.embargoed.fl_str_mv | 2022-02-21T18:00:39Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Cal, Pedro M.S.D. Sieglitz, Florian Santos, Fábio Parente Carvalho, Cátia Guerreiro, Ana Bertoldo, Jean B. Pischel, Uwe Gois, Pedro M. P. Bernardes, Gonçalo J. L. |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-02-21T18:00:39Z |
| dc.date.embargoed.fl_str_mv | 2022-02-21T18:00:39Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/51441 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/f73d1645-6985-446f-9377-7ef045612de6/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | OE |
| id | ul_98faada4ec84bcc0575bb8d0728e53bc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/51441 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/51441 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Cal, Pedro M.S.D. Cal, Pedro M.S.D. https://www.ciencia-id.pt/D113-1316-F38D D113-1316-F38D http://orcid.org/0000-0002-2512-7038 0000-0002-2512-7038 Sieglitz, Florian Sieglitz, Florian http://orcid.org/0000-0003-4480-8450 0000-0003-4480-8450 Santos, Fábio Santos, Fábio https://www.ciencia-id.pt/8C1F-2DFC-4A67 8C1F-2DFC-4A67 http://orcid.org/0000-0002-9057-9276 0000-0002-9057-9276 Parente Carvalho, Cátia Guerreiro, Ana Guerreiro, Ana https://www.ciencia-id.pt/481C-A494-B69D 481C-A494-B69D http://orcid.org/0000-0002-2173-6514 0000-0002-2173-6514 Bertoldo, Jean B. Pischel, Uwe Gois, Pedro M. P. Gois, Pedro M. P. https://www.ciencia-id.pt/AD1B-6A14-C952 AD1B-6A14-C952 http://orcid.org/0000-0002-7698-630X 0000-0002-7698-630X Bernardes, Gonçalo J. L. Bernardes, Gonçalo J. L. http://orcid.org/0000-0001-6594-8917 0000-0001-6594-8917 |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engRoyal Society of Chemistrypt_PTFluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.application/pdfpt_PTSite-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cellsPersonalCal, Pedro M.S.D.DSpacehttp://dspace.org/items/40b683a2-d878-491a-92d6-cd0044708acbDSpacehttp://dspace.org/items/40b683a2-d878-491a-92d6-cd0044708acbCalPedroCiência IDhttps://www.ciencia-id.ptD113-1316-F38DORCIDhttp://orcid.org0000-0002-2512-7038PersonalSieglitz, FlorianDSpacehttp://dspace.org/items/7f6cb48c-5883-4aa1-8a3e-c52a33aa0708DSpacehttp://dspace.org/items/7f6cb48c-5883-4aa1-8a3e-c52a33aa0708SieglitzFlorianORCIDhttp://orcid.org0000-0003-4480-8450PersonalSantos, FábioDSpacehttp://dspace.org/items/c3093147-7fe2-40e4-aded-f148e67ea23eDSpacehttp://dspace.org/items/c3093147-7fe2-40e4-aded-f148e67ea23eSantosFábioCiência IDhttps://www.ciencia-id.pt8C1F-2DFC-4A67ORCIDhttp://orcid.org0000-0002-9057-9276Researcher IDhttps://www.researcherid.comB-7028-2016Scopus Author IDhttps://www.scopus.com55645426300Parente Carvalho, CátiaPersonalGuerreiro, AnaDSpacehttp://dspace.org/items/1c4166db-cf53-48ab-9e3f-476f142cb304DSpacehttp://dspace.org/items/1c4166db-cf53-48ab-9e3f-476f142cb304dos Santos GuerreiroAna MariaCiência IDhttps://www.ciencia-id.pt481C-A494-B69DORCIDhttp://orcid.org0000-0002-2173-6514Bertoldo, Jean B.Pischel, UwePersonalGois, Pedro M. P.DSpacehttp://dspace.org/items/924d6caf-9ad2-444a-9926-cd2776d996adDSpacehttp://dspace.org/items/924d6caf-9ad2-444a-9926-cd2776d996adGoisPedroCiência IDhttps://www.ciencia-id.ptAD1B-6A14-C952ORCIDhttp://orcid.org0000-0002-7698-630XResearcher IDhttps://www.researcherid.comA-9251-2014Scopus Author IDhttps://www.scopus.com6507726637PersonalBernardes, Gonçalo J. L.DSpacehttp://dspace.org/items/d1a48067-77b1-4413-b1c7-602fb18c62c0DSpacehttp://dspace.org/items/d1a48067-77b1-4413-b1c7-602fb18c62c0BernardesGonçaloORCIDhttp://orcid.org0000-0001-6594-8917Scopus Author IDhttps://www.scopus.com14046757500HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1359-7345DOIIsPartOf10.1039/C6CC08671C2022-02-21T18:00:39Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/51441http://purl.org/coar/access_right/c_abf2open access2084994 bytesFundação para a Ciência e a TecnologiaIMINOBORONATES AS USEFUL SCAFFOLDS FOR TUMOR-TARGETINGCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaA “Click and CO Release” approach for the targeted delivery of therapeutic CO in vivoOECrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2017http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/f73d1645-6985-446f-9377-7ef045612de6/downloadChemical Communications532368371 |
| spellingShingle | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells Cal, Pedro M.S.D. |
| status | SINGLETON |
| title | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| title_full | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| title_fullStr | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| title_full_unstemmed | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| title_short | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| title_sort | Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells |
| url | http://hdl.handle.net/10451/51441 |
| visible | 1 |